Modifying Hydrolases to Catalyze New Bond Formation Reactions

修饰水解酶以催化新键形成反应

• 批准号: 0616560
• 负责人: Romas Kazlauskas
• 金额: --
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0616560&HistoricalAwards=false
• 关键词: Modifying; Hydrolases; Catalyze; New; Bond

This project will attempt to modify biocatalysts so that they can catalyze industrially relevant reactions. The first objective is to identify and extend the essential catalytic elements in epoxide hydrolases in order for them to accept external nucleophiles. Ability of the epoxide hydrolases to accept external nucleophiles could lead to non-natural but synthetically useful reactions. The second objective is to identify and extend the essential catalytic elements in hydroxynitrile lyase in order for them to accept carbon nucleophiles other than cyanide. This objective could also lead to non-natural but synthetically useful reactions.With this award, the Organic and Macromolecular Chemistry Program is supporting the research of Professor Romas J. Kazlauskas of the Department of Biochemistry at University of Minnesota-Twin Cities. Professor Kazlauskas's research efforts revolve around the modification of biocatalysts to catalyze non-natural but synthetically useful reactions. Such reactions are environmentally friendly, as they reduce costs as well as pollution. These reactions could improve the preparation of fine chemicals, which are needed in chemical and pharmaceutical industries.

该项目将尝试修改生物催化剂，以便它们可以催化与工业相关的反应。 第一个目标是识别和扩展环氧水解酶中必需的催化元素，以便它们接受外部亲核试剂。 环氧化物水解酶接受外核试剂的能力可能导致非天然但合成有用的反应。 第二个目标是识别和扩展羟基硝基裂解酶的基本催化元素，以便它们接受氰化物以外的碳接核剂。 这一目标还可能导致非自然但合成有用的反应。在该奖项中，有机和大分子化学计划正在支持明尼苏达大学 - 两国城市生物化学系罗马·卡兹劳斯卡斯教授的研究。 Kazlauskas教授的研究工作围绕着生物催化剂的修饰，以催化非天然但合成有用的反应。 这种反应对环境友好，因为它们降低了成本和污染。 这些反应可以改善化学和制药行业所需的精细化学物质的制备。