Evolution of Somatic Sex Determination in Nematodes

线虫体细胞性别决定的进化

• 批准号: 0615996
• 负责人: Andre Pires da Silva
• 金额: --
• 依托单位: University of Texas at Arlington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0615996&HistoricalAwards=false
• 关键词: Evolution; Somatic; Sex; Determination; Nematodes

Principal Investigator: Andre Pires da SilvaProposal Number: 0615996Title: Evolution of somatic sex determination in nematodesOne of the aspects that seems to change very fast in evolution is the genetic mechanism that determines the sex of the animal. This is a proposal to study the genetic basis of sexual dimorphism in the nematode Pristionchus pacificus. The main goal is to functionally compare well-characterized sex determination gene homologues of the nematode Caenorhabditis elegans with P. pacificus. The study of related species should provide important information to understand how rapidly evolving pathways occur. This proposal makes use of the recently sequenced genome of P. pacificus and newly developed genetic technologies in this species. Genomic searches of Pristionchus genomes have identified five homologous genes involved in sex determination in C. elegans. Using a combination of genetic techniques, the function of these genes will be tested in P. pacificus. In parallel, sex determination mutants will be induced in this species. Although the identity of the genes involved in P. pacificus sex determination is difficult to predict, it is likely that they will be different from C. elegans. In this case, such fundamental questions as where these different genes come from, when were they integrated into the sex determination pathway and what is their function in C. elegans can be addressed. This research has a broad impact because will integrate research and education by involving undergraduate and graduate students, including different genders and underrepresented minorities. Furthermore, insights about mechanisms of sex determination in different nematodes can have practical value for sterilization of animal and plant parasites.

首席研究员：Andre Pires DA Silvaproposal编号：0615996TITLE：在神经酮中的体细胞性别确定的进化，这些方面似乎在进化中迅速变化是决定动物性别的遗传机制。这是研究线虫pristionchus pacificus中性二态性的遗传基础的一项建议。主要目的是在功能上比较秀丽隐杆线虫与太平洋假单胞菌的良好特征性的性别确定基因同源物。 对相关物种的研究应提供重要信息，以了解如何快速发展的途径。该建议利用了最近测序的太平洋假单胞菌和该物种新开发的遗传技术的基因组。 Pristionchus基因组的基因组搜索已经鉴定出五个参与秀丽隐杆线虫性别确定的同源基因。结合遗传技术，将在Pacificus中测试这些基因的功能。同时，将在该物种中诱导性别确​​定突变体。尽管很难预测到太平洋性性别性别性别的基因的身份，但它们可能与秀丽隐杆线虫不同。在这种情况下，这些不同的基因来自何处，何时将它们整合到性别确定途径以及它们在秀丽隐杆线虫中的功能是什么。这项研究具有广泛的影响，因为将通过涉及本科生和研究生（包括不同的性别和代表性不足的少数民族）来整合研究和教育。此外，关于不同线虫中性别确定机制的见解可能对动物和植物寄生虫的灭菌具有实际价值。