SBIR Phase I: Temperature-independent SPR Biosensor

SBIR 第一阶段：与温度无关的 SPR 生物传感器

• 批准号: 0610871
• 负责人: Paul Melman
• 金额: $ 10万
• 依托单位: Newton Photonics, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0610871&HistoricalAwards=false
• 关键词: SBIR; Phase; Temperature; independent; SPR

This Small Business Innovation Research (SBIR) Phase I project will demonstrate the feasibility of a temperature-independent surface plasmon resonance (SPR) biosensor for sensitive detection of biological molecules. SPR bio-detection has been used for many years in biomedical research and drug development laboratories. Recent improvement in sensitivity and potentially lower cost enable the use of SPR-based biosensor in diagnostic application, including testing of pathogens, biomarkers, toxins and contaminants. However, current SPR instruments are susceptible to temperature-induced measurement errors that limit their use in the field. The thermal drift in SPR sensors is caused by the dependence of the refractive index of the sensor's optical medium and the sample material on ambient temperature. Currently, this thermal drift is mitigated in laboratory instruments by stabilizing the temperature in the instrument's test chamber and by incorporating temperature compensation channels into the sensor design. However, these active measures increase the instrument's complexity, cost, size and power consumption. The proposed temperature-independent SPR design addresses this fundamental deficiency and reduces by a factor of 100~1000 the thermal sensitivity of a waveguide-based SPR chip by matching the thermo-optic coefficients of the chip's optical substrate and the sample under test. If successful the proposed project will lead to expansion of the application range and market penetration of SPR biodetection technology. Based on the high-sensitivity and low cost of the proposed SPR sensor, a handheld instrument will be developed to support multiple field and point-of-care diagnostic applications in the areas of emergency medicine, veterinary medicine, food safety, aquaculture and biodefense.

这项小型企业创新研究（SBIR）I期项目将证明与温度无关的表面等离子体共振（SPR）生物传感器的可行性，用于对生物分子的敏感检测。 SPR生物检测已在生物医学研究和药物开发实验室中使用了多年。最近的敏感性和潜在降低成本的提高，可以在诊断应用中使用基于SPR的生物传感器，包括病原体，生物标志物，毒素和污染物的测试。但是，当前的SPR仪器容易受到限制其在现场使用的温度引起的测量误差的影响。 SPR传感器中的热漂移是由传感器光学介质的折射率和样品材料对环境温度的依赖性引起的。目前，通过稳定仪器测试室中的温度并将温度补偿通道纳入传感器设计，可以在实验室仪器中减轻这种热漂移。但是，这些主动措施增加了仪器的复杂性，成本，大小和功耗。提出的独立于温度的SPR设计解决了这种基本缺陷，并通过与芯片光学底物的热光系数和测试的样品相匹配，从而使基于波导的基于波导的SPR芯片的热灵敏度减少了100〜1000。如果成功，提议的项目将导致SPR生物调节技术的应用范围和市场渗透扩大。根据拟议的SPR传感器的高敏性和低成本，将开发手持仪器，以支持急诊医学，兽医医学，食品安全，水产养殖和生物养殖领域的多个领域和护理诊断应用。