Collaborative Research: Genetic Basis of Behavioral Evolution in Zebrafish

合作研究：斑马鱼行为进化的遗传基础

• 批准号: 0543491
• 负责人: Emilia Martins
• 金额: --
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0543491&HistoricalAwards=false
• 关键词: Collaborative; Research; Genetic; Basis; Behavioral

I. Intellectual Merit:Behavioral differences between natural populations of zebrafish will be conducted to lay the groundwork for future use of this model organism to answer questions about the genetic basis of behavioral evolution. Investigators will travel to India to conduct preliminary investigations of zebrafish natural habitats, identifying environmental features that may be particularly important to behavioral diversification. Wild-caught fish from two populations will be established as new strains in the lab and behaviorally characterized. Behavioral differences between wild-type strains will be compared to differences already observed between existing laboratory strains of zebrafish in general activity, social behavior (shoaling and aggression), predator response (surface orientation, proximity to predator), and stress response (startle behavior and recovery from disturbance). The results will set the stage for future genetic research using crosses and genomic mapping techniques to determine whether similar behavioral differences are associated with similar genetic changes. II. Broader Impact:This project will begin to produce and disseminate a collection of behavioral protocols for other researchers (e.g., geneticists, developmental biologists). Researchers will also continue to work with a team of high-school teachers to develop and to test inquiry-oriented curricular materials based on zebrafish behavioral genetics. Two new wild-type strains of zebrafish will be established and contributed to the Zebrafish Stock Center.

I.智力价值：将研究斑马鱼自然种群之间的行为差​​异，为将来使用该模式生物回答有关行为进化的遗传基础的问题奠定基础。研究人员将前往印度对斑马鱼的自然栖息地进行初步调查，确定对行为多样化可能特别重要的环境特征。来自两个种群的野生捕获的鱼将在实验室中建立为新品种并进行行为特征分析。野生型品系之间的行为差​​异将与现有斑马鱼实验室品系之间已经观察到的差异进行比较，这些差异包括一般活动、社会行为（成群和攻击）、捕食者反应（表面方向、与捕食者的接近程度）和应激反应（惊吓行为和攻击行为）。从干扰中恢复）。这些结果将为未来使用杂交和基因组作图技术的遗传研究奠定基础，以确定相似的行为差异是否与相似的遗传变化相关。二. 更广泛的影响：该项目将开始为其他研究人员（例如遗传学家、发育生物学家）制作和传播一系列行为协议。研究人员还将继续与高中教师团队合作，开发和测试基于斑马鱼行为遗传学的探究型课程材料。将建立两个新的野生型斑马鱼品系并将其贡献给斑马鱼库存中心。