Ultrashortloop Feedback on GnRH Biosynthesis and Secretion

GnRH 生物合成和分泌的超短环反馈

• 批准号: 0544150
• 负责人: Tao-Yiao Wu
• 金额: $ 30.08万
• 依托单位: Henry M Jackson Fdn for Advmt of Military Medicine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0544150&HistoricalAwards=false
• 关键词: Ultrashortloop; Feedback; GnRH; Biosynthesis; Secretion

The decapeptide gonadotropin-releasing hormone (GnRH) is the central regulator of reproductive function and behavior in mammals. It is secreted from the hypothalamus to induce the release of gonadotropins, which in turn, stimulate gonadal function. Recently it has been discovered that the metabolite of GnRH, the pentapeptide, GnRH-(1-5), can directly regulate its gene expression as well as to facilitate reproductive behavior in the ovariectomized estrogen-primed rat. These results have led to the overarching hypothesis that this metabolite of GnRH, GnRH-(1-5) may be important for the synchronization of the preovulatory GnRH/LH surge with the initiation of reproductive behavior in the rodent. The studies in the current project will provide a fundamental understanding for how this metabolite of GnRH, GnRH-(1-5), is physiologically important for the regulation of pulsatile GnRH secretion. The experiments are designed to enhance our understanding of the cellular and molecular mechanisms underlying this processed metabolite's action in the brain. It is anticipated that this project will involve a graduate student, 1-2 rotating graduate student(s), and at least 5 college or high school students. The high school/college students will participate through summer programs or chapters of science/educational foundations as well as local undergraduate institutions. The high school/college projects will involve the central injections of peptides or inhibitors. The students will be involved in data presentation at weekly meetings as well as the publication process.

脱肽促性激素释放激素（GNRH）是哺乳动物生殖功能和行为的中心调节剂。 从下丘脑诱导促性腺激素的释放，这又刺激了性腺功能。 最近，已经发现，GnRH的代谢产物，五肽GnRH-（1-5）可以直接调节其基因表达，并促进卵巢雌激素传染的大鼠的生殖行为。 这些结果导致了总体假设，即这种GnRH，GnRH-（1-5）的代谢产物对于在啮齿动物中的生殖行为的启动时，对卵巢前GNRH/LH激增的同步可能很重要。 当前项目中的研究将对GnRH的代谢产物GnRH-（1-5）的代谢产生基本了解在生理上对调节脉冲GnRH分泌的重要性很重要。 这些实验旨在增强我们对这种加工代谢物在大脑中作用的细胞和分子机制的理解。 预计该项目将涉及一名研究生，1-2个旋转的研究生，以及至少5名大学或高中生。 高中/大学生将通过夏季课程或科学/教育基金会以及当地的本科机构参加。高中/大学项目将涉及肽或抑制剂的中心注射。 学生将在每周会议和出版过程中参与数据演示。