MRI/RUI Acquisition of a Liquid Chromatograph-Mass Spectrometer to Support Chemistry Research at California State University, Northridge

MRI/RUI 采购液相色谱-质谱仪以支持加州州立大学北岭分校的化学研究

• 批准号: 0521688
• 负责人: Eric Kelson
• 金额: $ 33.67万
• 依托单位: The University Corporation, Northridge
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0521688&HistoricalAwards=false
• 关键词: MRI; RUI; Acquisition; Liquid; Chromatograph

With this award from the Major Research Instrumentation (MRI) Program, the Department of Chemistry at California State University in Northridge will acquire a liquid chromatograph ion trap tandem mass spectrometer (LC/MS). Five areas of research will immediately benefit from the requested LC-MS instrumentation: (1) Direct design of transfer hydrogenation catalysts. LC-MS will be used in confirmation of key reaction intermediates (especially ruthenium hydrides and catalyst-substrate adducts) as well as characterization of new catalysts and the products they produce. (2) Understanding fundamental molecular processing in organic sonochemistry. LC-MS will facilitate the determination of intermediate structures from thermal and hydroxyl radical pathways and distinction of the underlying mechanisms. (3) Aromatase-induced metabolism of poly-functional 1,5-alkadiyne anti-tumor agents. LC-MS will be important to confirm aromatase-drug interactions, identify metabolites and test pro-drug designs for targeted delivery of promising agents in breast cancer. (4) Simultaneous analysis of mRNA and protein. LC-MS will be used in the separation and identification of target proteins (beginning with estrogen receptor in breast cancer cells) from bulk lysate samples. (5) Evaluation of the extent and sites of apolipoprotein E glycation. LC-MS will be used to confirm fructoselysine residues within proteolytic fragments of apolipoprotein E secreted from THP-1 macrophages at elevated glucose concentrations. Liquid chromatography with mass spectrometric detection (LC/MS) is an extremely powerful technique used for the separation and analysis of complex mixtures. This instrument will substantially strengthen scientific research at California State University in Northridge, a primarily undergraduate institution. These studies will have a solid impact in the area of biochemistry and medicinal chemistry.

凭借重大研究仪器 (MRI) 计划的这一奖项，北岭加州州立大学化学系将获得一台液相色谱离子阱串联质谱仪 (LC/MS)。五个研究领域将立即受益于所需的 LC-MS 仪器：（1）转移氢化催化剂的直接设计。 LC-MS 将用于确认关键反应中间体（特别是氢化钌和催化剂-底物加合物）以及新催化剂及其产生的产品的表征。 (2) 了解有机声化学中的基本分子处理。 LC-MS 将有助于确定热和羟基自由基途径的中间结构，并区分潜在机制。 (3)芳香酶诱导的多功能1,5-二炔抗肿瘤剂的代谢。 LC-MS 对于确认芳香酶-药物相互作用、识别代谢物和测试前药设计以靶向递送有前景的乳腺癌药物非常重要。 (4) mRNA和蛋白质的同时分析。 LC-MS 将用于从大量裂解物样品中分离和鉴定目标蛋白（从乳腺癌细胞中的雌激素受体开始）。 (5)载脂蛋白E糖化程度和位点的评价。 LC-MS 将用于确认在升高的葡萄糖浓度下 THP-1 巨噬细胞分泌的载脂蛋白 E 的蛋白水解片段中的果糖赖氨酸残基。液相色谱与质谱检测 (LC/MS) 是一种用于分离和分析复杂混合物的极其强大的技术。该仪器将大大加强位于北岭的加州州立大学（主要是本科院校）的科学研究。 这些研究将对生物化学和药物化学领域产生重大影响。