Ecology of a Distinct Bdellovibrio Genotype in the Chesapeake Bay and other estuaries

切萨皮克湾和其他河口独特的蛭弧菌基因型的生态学

• 批准号: 0455276
• 负责人: Henry Williams
• 金额: --
• 依托单位: Florida Agricultural and Mechanical University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-10-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0455276&HistoricalAwards=false
• 关键词: Ecology; Distinct; Bdellovibrio; Genotype; Chesapeake

In a previous NSF project (OCE 0084265) the investigators discovered a distinct (d) halophilic (H) Bdellovibrionaceae (Bd) genotype that appears to be exclusive to the Chesapeake Bay estuary. This is the first time a distinct population of Bd that appears to be restricted to a particular ecosystem has been reported. Although some ocean isolates were found in the Bay, it appears that the unique Bay genotype is a distinct strain that has adapted to the estuarine environment. The current project will follow-up on this finding by addressing the following questions: 1) are the unique genotypes recovered from the Chesapeake Bay exclusive to that body or are they common to other estuaries along the Atlantic coast; 2) do Bay genotypes occur primarily in the Bay proper or in the smaller more protected sub estuaries of the Bay; 3) do the bacteria in the Chesapeake Bay and its sub estuaries and other estuaries select for the unique Bd genotypes; 4) can other distinct genotypes of Bd be detected in the Bay using non-culture methods? The investigators will address these questions by conducting a spatial and temporal sampling program, examining predation patterns by Bd on bacteria, and using molecular methods to examine genotypes of Bd forms. The broader impacts of this activity are that it will integrate the research into education and, in particular, will seek to broaden the participation of underrepresented groups. During the three years of the project the investigators expect to train no less than 15 students and teachers. Based on previous experience more than half will be members of underrepresented groups. The research project will involve the use of basic microbiology and state of the art molecular techniques sequencing, cloning, amplification using PCR and quantification using real time PCR and will provide valuable training in modern microbial techniques to participating students and teachers. These activities will expose and encourage more individuals to pursue careers in sciences and thereby contribute to meeting the nations need for a highly trained technical workforce.

在之前的 NSF 项目 (OCE 0084265) 中，研究人员发现了一种独特的 (d) 嗜盐 (H) 蛭弧菌科 (Bd) 基因型，该基因型似乎是切萨皮克湾河口独有的。这是第一次报道似乎仅限于特定生态系统的独特 Bd 种群。尽管在海湾中发现了一些海洋分离株，但独特的海湾基因型似乎是一种适应河口环境的独特菌株。 当前的项目将通过解决以下问题来跟进这一发现：1）从切萨皮克湾回收的独特基因型是该机构独有的，还是大西洋沿岸其他河口所共有的？ 2) 海湾基因型主要发生在海湾本身还是较小的、更受保护的海湾亚河口； 3）切萨皮克湾及其子河口和其他河口的细菌是否会选择独特的Bd基因型？ 4) 使用非培养方法可以在海湾中检测到其他不同的 Bd 基因型吗？ 研究人员将通过执行空间和时间采样计划、检查 Bd 对细菌的捕食模式以及使用分子方法检查 Bd 形式的基因型来解决这些问题。 这项活动更广泛的影响在于它将研究融入教育，特别是寻求扩大代表性不足群体的参与。在该项目的三年期间，研究人员预计将培训不少于 15 名学生和教师。根据以往的经验，一半以上将是代表性不足的群体的成员。该研究项目将涉及使用基础微生物学和最先进的分子技术测序、克隆、PCR 扩增和实时 PCR 定量，并将为参与的学生和教师提供现代微生物技术的宝贵培训。 这些活动将揭露并鼓励更多的人从事科学事业，从而有助于满足国家对训练有素的技术劳动力的需求。