U.S.-France Cooperative Research: Energetics and Conformational Changes of SNARE-mediated Fusion

美法合作研究：SNARE介导的融合的能量学和构象变化

基本信息

批准号：
0437230
负责人：
Thomas Melia
金额：
$ 2.2万
依托单位：
Columbia University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2005
资助国家：
美国
起止时间：
2005-05-01 至 2007-04-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0437230&HistoricalAwards=false
关键词：
U.S.France Cooperative Research Energetics

项目摘要

In this two-year U.S.-France cooperative research project, the investigators will address questions related to SNARE proteins and membrane fusion mechanisms in cells. SNARE proteins will be incorporated into lipid bilayers or expressed on cell membranes. Energy measurements will be performed using several nano techniques that allow force and energy measurements between macromolecular assemblies. The combination of these techniques will allow the investigators to address: (1) the number of SNARE complexes involved in membrane fusions; (2) force versus time measurements and a kinetic description of SNARE-pin formation; and (3) evaluation of the energy associated to the SNARE-pin formation and the amount of energy necessary for membrane fusion. This project involves Thomas Melia at Columbia University and researchers at the Laboratoire de Physique Statistique at the Ecole Normale Superieure in Paris, France. The laboratories have complementary expertise and available equipment. The Columbia group specializes in SNARE proteins and their role in membrane fusion. This is complemented by French expertise in biological adhesion mechanisms and nanotechniques that allow force and energy measurements between biomembranes.

在这个为期两年的美法合作研究项目中，研究人员将解决与 SNARE 蛋白和细胞膜融合机制相关的问题。 SNARE 蛋白将被整合到脂质双层中或在细胞膜上表达。能量测量将使用多种纳米技术进行，这些技术允许测量大分子组装体之间的力和能量。这些技术的结合将使研究人员能够解决：（1）参与膜融合的 SNARE 复合物的数量； (2) 力与时间的关系测量以及 SNARE-pin 形成的动力学描述； (3) 评估与 SNARE-pin 形成相关的能量和膜融合所需的能量。该项目由哥伦比亚大学的 Thomas Melia 和法国巴黎高等师范学院物理统计实验室的研究人员参与。这些实验室拥有互补的专业知识和可用的设备。哥伦比亚小组专门研究 SNARE 蛋白及其在膜融合中的作用。法国在生物粘附机制和纳米技术方面的专业知识对此进行了补充，这些技术可以测量生物膜之间的力和能量。