Collaborative Research: Determination of Oceanic Copper and Zinc using Biosensor Technology

合作研究：利用生物传感器技术测定海洋铜和锌

• 批准号: 0425529
• 负责人: Carol Fierke
• 金额: $ 12.34万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0425529&HistoricalAwards=false
• 关键词: Collaborative; Research; Determination; Oceanic; Copper

The PIs propose to develop fluorescence-based fiber optic biosensors designed to measure oceanic trace levels of free Zn2+ and Cu2+ continuously in situ, in real time. These biosensors use a biomolecule, carbonic anhydrase, to transduce the level of free metal ion as a change in fluorescence intensity or lifetime that can be measured through a length of optical fiber. The use of the biomolecule as transducer confers unmatched selectivity and sensitivity on these optical sensors. Proposed improvements in the transducer molecule, fluorescent labels, and optics will permit measurement deep in the water column and in anoxic waters, where current analytical technology is incapable of acquiring the needed data. This capability will permit questions of central importance in surface zinc bioavailability, Cu availability for methanotrophs and denitrifiers, and Cu availability in reducing conditions to finally be addressed. A key goal of this project is the training of students and the introduction of students outside the discipline to marine biogeochemistry and the special character of research in a shipboard environment.

PI 建议开发基于荧光的光纤生物传感器，旨在连续、实时地原位测量海洋痕量游离 Zn2+ 和 Cu2+ 水平。这些生物传感器使用生物分子碳酸酐酶将游离金属离子的水平转换为荧光强度或寿命的变化，可以通过一段光纤进行测量。使用生物分子作为传感器赋予这些光学传感器无与伦比的选择性和灵敏度。对传感器分子、荧光标记和光学器件的拟议改进将允许在水柱深处和缺氧水域进行测量，而当前的分析技术无法获取所需的数据。这种能力将最终解决表面锌生物利用率、甲烷氧化菌和反硝化菌的铜利用率以及还原条件下的铜利用率等核心问题。该项目的一个关键目标是培训学生并向学科外的学生介绍海洋生物地球化学以及船上环境研究的特殊性。