Somatic Karyotype Analysis of the Maize Genome

玉米基因组的体细胞核型分析

• 批准号: 0423898
• 负责人: James Birchler
• 金额: --
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0423898&HistoricalAwards=false
• 关键词: Somatic; Karyotype; Analysis; Maize; Genome

The project will develop a method to visually identify each of the ten chromosomes of maize. A set of probes that localize to specific sites on each chromosome and that produce a specific pattern of fluorescence will be generated. Once this method is optimized, the variation of karyotypes (chromosome images) among diverse maize germplasm and closely related species will be determined. The types of organelle genome transfer to the nucleus in these materials will be established. The karyotyping system will be used to examine the nature of genome size variation in hybrid plants by determination of changes in quantity of repetitive sequence arrays at different locations in the genome. Primed in situ labeling coupled with polymerase chain reaction amplification will be adapted for maize chromosomes and used to test the ability to detect specific subclasses of repeated sequences as well as the visualization of individual genes and introduced genes. Chromosome painting probes will be developed from diverse sources and used to label the chromosomes with multiple colors as an aid to detection of subtle changes in chromosome structure. The development of a robust chromosome identification system will enhance studies of maize genomics. With the ability to identify each chromosome, determinations of changes in chromosome number and structure can be easily made. Investigations that examine chromosome behavior or rearrangement will be greatly facilitated. Variations in gene structure in different lines of maize can be visualized using primed in situ polymerase chain reaction amplification. The completion of this project will reveal new knowledge about chromosome structure, organelle genome transfer, the behavior and variation of repetitive sequences and the evolution of the genome. Access to project outcomesThe results will be presented in publications and a project website that will be linked to the MaizeGDB database.

该项目将开发一种方法，以视觉上识别玉米的十种染色体中的每一个。将生成一组定位于每个染色体上特定位点并产生特定荧光模式的探针。一旦优化了该方法，将确定各种玉米种质和密切相关的物种之间核型（染色体图像）的变化。将建立细胞器基因组转移到这些材料中的细胞核的类型。核分型系统将通过确定基因组不同位置的重复序列阵列数量的变化来检查杂交植物中基因组大小变化的性质。与聚合酶链反应扩增结合的原位标记，将适用于玉米染色体，并用于测试检测重复序列的特定亚类以及单个基因和引入基因的可视化能力。染色体绘画探针将从各种来源开发，并用来标记具有多种颜色的染色体，以帮助检测染色体结构的细微变化。强大的染色体鉴定系统的发展将增强对玉米基因组学的研究。有了识别每个染色体的能力，可以轻松地确定染色体数和结构的变化。检查染色体行为或重排的调查将大大促进。可以使用原位聚合酶链反应放大的原位聚合酶链反应可视化基因结构的变化。该项目的完成将揭示有关染色体结构，细胞器基因组转移，重复序列的行为和变异以及基因组进化的新知识。将访问项目的访问结果将在出版物和将链接到玉米GDB数据库的项目网站中介绍。