A Functional Genomics Approach to Investigate Regulation of Phenolic Glycoside Metabolism in Populus

研究杨树酚苷代谢调节的功能基因组学方法

• 批准号: 0421756
• 负责人: Chung-Jui Tsai
• 金额: $ 211.41万
• 依托单位: Michigan Technological University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-11-15 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421756&HistoricalAwards=false
• 关键词: Functional; Genomics; Approach; Investigate; Regulation

There is a wealth of evidence that the metabolic cost of defense is large in comparison to resources available for growth in plants. The abundant phenolic glycosides (PG) of Populus tree species are derived from salicylic acid, a metabolite that is central to the defense of herbaceous and woody plant species, but whose accumulation is extremely costly to herbaceous plant growth. By investigating the functional genomics of PG regulation in a range of hybrid and transgenic Populus, we expect to learn how these plants accommodate such costly outlays, and to identify genes of potential agricultural importance. Cottonwood hybrids varying widely in leaf PG content and growth will be used to obtain differentially expressed PG-regulating and PG-sensitive genes. PG inductive treatments including nitrogen limitation and methyl-jasmonate application will be used to perturb PG homeostasis in the hybrids as well as in transgenically modified aspen, and to verify gene function and assess PG-growth interactions. EST sequences and microarray gene expression profiles derived from this work will be deposited in GenBank and accessible at www.aspenDB.mtu.edu. cDNA microarrays will also be made available to the research community. Information about the release schedule and fee structure of ESTs, arrays, and other biological resources will be posted on the project website at aspenDB.mtu.edu. Project findings will bear particular relevance to the NPGI goal of understanding efficiency of photosynthate utilization in a renewable resource.There are several anticipated broader impacts. A deeper understanding of the molecular basis of tree carbon budgeting should be of value to ongoing investigation of Populus as keystone ecological species whose productivity and phytochemisty is particularly sensitive to global changes in atmospheric pollutants and greenhouse gases. There are also agricultural crop implications due to possible interplay between PG and condensed tannin (CT) regulation, and the effects of CT on forage digestibility/quality. The value of Populus as a model organism for linking functional genomics to ecological research will be disseminated via a professional microarray workshop targeting tree physiologists and ecophysiologists in year 4. Because aspen is important to the regional economy its use in the planned K-12 educational activities is particularly relevant. Planned outreach activities will emphasize the significance and value of basic research to participating students and their families.

有很多证据表明，与可用于植物生长的资源相比，国防代谢成本很大。 群树的丰富酚类糖苷（Pg）源自水杨酸，水杨酸是一种代谢物，是防御草本和木质植物物种的核心，但其积累对草本植物植物的生长非常昂贵。 通过研究PG调节的功能基因组学在一系列杂种和转基因人群中，我们希望学习这些植物如何适应这种昂贵的支出，并确定潜在的农业重要性基因。 在叶片PG含量和生长中，杨木杂种将广泛变化，以获取差异表达的PG调节和PG敏感基因。 PG电感治疗包括氮的限制和甲基贾斯酯的施用将用于扰动杂种以及转基因的Aspen中的PG稳态，并验证基因功能并评估PG-GROWTH相互作用。 EST序列和微阵列基因表达曲线得出的这项工作将沉积在GenBank中，可在www.aspendb.mtu.edu上访问。 cDNA微阵列也将提供给研究社区。 有关EST，阵列和其他生物资源的发布时间表和费用结构的信息将发布在项目网站aspendb.mtu.edu上。 项目发现将与可再生资源中光合作用利用的效率的NPGI目标特别相关。有几种预期的更广泛的影响。对树碳预算的分子基础的更深入的理解对于对Populus作为Keystone生态物种的持续研究应该具有价值，其生产力和植物化学物质对大气污染物和温室气体的全球变化特别敏感。 由于PG和凝结的单宁（CT）调节之间的可能相互作用以及CT对草料消化率/质量的影响，也存在农作物作物的影响。 Populus作为模型生物学对于将功能基因组学与生态研究联系起来的价值将通过第4年的专业微阵列研讨会来传播，该研讨会针对树生理学家和生态生理学家。计划的外展活动将强调基础研究对参与学生及其家人的重要性和价值。