DISSERTATION RESEARCH: Hybridization, Polyploidy and Pollinator Behavior in Indian Paintbrush (Castilleja).

论文研究：印度画笔（Castilleja）的杂交、多倍体和传粉者行为。

基本信息

批准号：
0412350
负责人：
Barbara Roy
金额：
$ 1.09万
依托单位：
University of Oregon Eugene
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2004
资助国家：
美国
起止时间：
2004-06-01 至 2007-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0412350&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Hybridization Polyploidy Pollinator

项目摘要

The goal of this project is to understand how hybridization and polyploidy, in tandem, influence observed phenotypic patterns in natural populations, and how pollinator behavior, through selection, reinforces such patterns. This study examines 3 species of Indian Paintbrush that co-occur. In some locations, however, hybridization has obscured morphological distinctions between the species while in other locations species do not appear to hybridize and remain phenotypically distinct. We hypothesize that hybridization occurs in populations where the 3 co-occurring species have identical ploidy levels. In other plant systems, differences in ploidy levels between species can create barriers to reproduction. Plants from replicates of each type of population will be genotyped with AFLP markers and ploidy level will be determined by flow cytometry and chromosome counts. Pollination experiments in the field will determine whether pollinators, as agents of selection, help produce or reinforce observed patterns. Hybridization and polyploidy have been instrumental in the evolution of most plant lineages, yet how these processes and selection together influence landscape patterns of phenotypic variability has rarely been studied. By studying these two processes together, a greater understanding of their respective roles in the evolution of diverse plant lineages will be achieved.

该项目的目标是了解杂交和多倍体如何同时影响自然种群中观察到的表型模式，以及传粉者行为如何通过选择强化这种模式。这项研究考察了同时出现的 3 种印度画笔。然而，在某些地方，杂交掩盖了物种之间的形态差异，而在其他地方，物种似乎没有杂交并保持表型不同。我们假设杂交发生在 3 个共存物种具有相同倍性水平的群体中。在其他植物系统中，物种之间倍性水平的差异可能会造成繁殖障碍。来自每种类型群体的重复的植物将使用 AFLP 标记进行基因分型，并且倍性水平将通过流式细胞术和染色体计数确定。田间授粉实验将确定授粉媒介作为选择媒介是否有助于产生或强化观察到的模式。杂交和多倍体在大多数植物谱系的进化中发挥了重要作用，但这些过程和选择如何共同影响表型变异的景观格局却很少被研究。通过共同研究这两个过程，将更好地了解它们各自在不同植物谱系进化中的作用。