Whole Cell and Regulatory Protein-Based Sensors in Bioanalysis

生物分析中基于全细胞和调节蛋白的传感器

• 批准号: 0416553
• 负责人: Sylvia Daunert
• 金额: $ 36万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0416553&HistoricalAwards=false
• 关键词: Whole; Cell; Regulatory; Protein; Based

Professor Sylvia Daunert of the University of Kentucky is supported by the Analytical and Surface Chemistry Program to continue work on a whole-cell bacteria-based sensing system. The project goals are two-fold: first, to make practical whole-cell sensors that are stable enough for storage and field use. The idea is to use lypohilization and/or spores in a microfluidic format on a compact-disc. Secondly, the PI will apply whole cells as sensors of signalling chemicals that cells use to "decide" things like antibiotic production, virulence and release of bioluminescence in a mode called "quorum sensing (QS)." Intervention in bacteria signalling has been proposed as an alternative to traditional antibiotic therapy. This proposal spans the areas of chemical signalling, sensing, and complex systems, with an emphasis on cutting edge technology. Students, including those from Appalachia, are trained in this highly interdisciplinary approach to sensing, an area of national need in combatting terrorism.

肯塔基大学的西尔维亚·道纳特（Sylvia Daunert）教授得到了分析和表面化学计划的支持，以继续在基于全细菌的传感系统上工作。 项目目标是两个方面：首先，制造实用的全细胞传感器，这些传感器足以存储和现场使用。 这个想法是在紧凑型碟片上使用微流体格式使用lypohilization和/或孢子。其次，PI将将全细胞应用于信号化学物质的传感器，以“决定”诸如抗生素产生，毒力和生物发光之类的东西，以“ Quorum Sensing（QS）”的模式。 已经提出了细菌信号的干预作为传统抗生素疗法的替代方法。 该建议涵盖了化学信号，传感和复杂系统的领域，重点是尖端技术。 包括来自阿巴拉契亚的学生在内的学生接受了这种高度跨学科的传感方法，这是对抗恐怖主义的国家需求。