International Research Fellowship Program: Determination of the Function of RoXaN I in Cellular and Rotavirus Biology

国际研究奖学金计划：确定 RoXaN I 在细胞和轮状病毒生物学中的功能

• 批准号: 0401866
• 负责人: Michelle Becker
• 金额: --
• 依托单位: Becker Michelle M
• 依托单位国家: 美国
• 项目类别: Fellowship
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0401866&HistoricalAwards=false
• 关键词: International; Research; Fellowship; Program; Determination

0401866BeckerThe International Research Fellowship Program enables U.S. scientists and engineers to conduct three to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad.This award will support a twelve-month research fellowship by Dr. Michelle M. Becker to work with Dr. Didier Poncet at Centre National de la Recherche Scientique in Gif-sur-Yvette, France.RoXaN I is a cellular protein with no known function, identified through its interaction with rotavirus nonstructural protein NSP3. RoXaN is found in both the nucleus and the cytoplasm and may move between these two cellular compartments, possibly to transport mRNA or regulate translation. These experiments are designed to test the hypothesis that the normal function of RoXaN is subverted during rotavirus infection to enhance viral replication and will also contribute new information about the mechanisms of rotavirus replication and viral control of cellular processes. Since RoXaN is present in both the cytoplasm and nucleus, but it does not contain a classical nuclear localization sequence or nuclear export sequence, the subcellular localization of RoXaN in uninfected and rotavirus-infected cells is being determined using confocal immunofluorescence microscopy. Deletion mutants of RoXaN are being used to define the protein domains necessary for proper subcellular localization. Results from these experiments will define the localization of RoXaN within the cell and the regions within the protein important for directing RoXaN to the correct cellular compartment. The effect of RoXaN on rotavirus processes will be examined in the presence and absence of RoXaN expression. Cellular expression of RoXaN mRNA is being deleted using RNA interference technology. The efficiency of rotavirus replication, translation of viral and cellular messages, and changes in viral replication patterns will be examined in cells expressing and not expressing RoXaN. These results will determine the role of RoXaN in rotavirus replication.The host lab has unique tools to examine the function of this fascinating and novel protein. The host and his colleagues are experts in studies of the viral manipulation of cellular physiology, and have the resources necessary to complete this project.

0401866BECKERTHE国际研究奖学金计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。该计划的奖项提供了共同研究的机会，并使用独特或补充的设施，专业知识和国外的实验条件。该奖项将支持米歇尔·M·贝克尔（Michelle M.非结构蛋白NSP3。在细胞核和细胞质中都发现了Roxan，并且可能在这两个细胞室之间移动，可能是用于运输mRNA或调节翻译。这些实验旨在检验假设Roxan在轮状病毒感染过程中颠覆以增强病毒复制的假设，并将为有关轮状病毒复制机制和细胞过程病毒控制机制提供新的信息。由于Roxan存在于细胞质和核中，但不包含经典的核定位序列或核输出序列，因此使用共焦免疫荧光显微镜确定Roxan在未感染和轮状病毒感染细胞中的Roxan的亚细胞定位。 Roxan的缺失突变体用于定义适当的亚细胞定位所需的蛋白质结构域。这些实验的结果将定义Roxan在细胞内的定位，而蛋白质中的区域对于将Roxan引导到正确的细胞室很重要。在存在和不存在Roxan表达的情况下，将检查Roxan对轮状病毒过程的影响。 ROXAN mRNA的细胞表达正在使用RNA干扰技术删除。轮状病毒复制的效率，病毒和细胞信息的翻译以及病毒复制模式的变化将在表达和不表达Roxan的细胞中检查。这些结果将确定Roxan在轮状病毒复制中的作用。宿主实验室具有独特的工具来检查这种迷人和新颖的蛋白质的功能。主持人及其同事是研究细胞生理学病毒操作的专家，并拥有完成该项目所需的资源。