The follicle stem cell lineage in telotrophic Tribolium oogenesis

端养谷盗卵子发生中的滤泡干细胞谱系

• 批准号: 164564755
• 负责人: Dr. Michael Schoppmeier
• 金额: --
• 依托单位: Department Biologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/164564755?language=en
• 关键词: follicle; stem; cell; lineage; telotrophic

The beetle Tribolium represents telotrophic ovaries, which differ fundamentally from the polytrophic ovary of Drosophila. To gain insights into the molecular mechanisms underlying telotrophic ovary organogenesis, oogenesis, and somatic stem cell biology, we propose to functionally analyze all Tribolium genes by RNAi. In Drosophila, the tight spatial and temporal association of germline stem cells and somatic stem cells makes is very difficult lo analyze the somatic stem cell lineage. In Tribolium, however, systemic RNAi in the pupal stage allows to functionally analyze the somatic stem cells lineage without interference from disturbed germ line development. Larval RNAi, on the other hand, allows analyzing these earlier processes, e.g. ovary organogenesis and the specification germline derived cell population. The proposed project will enhance our knowledge on the development and evolution of different ovary types. Moreover, with the iBeetle screen we will for the first time identify a comprehensive set of genes required for ovary development and somatic stem cell biology, which has not been possible in Drosophila yet. As we expect principle mechanisms controlling stem cell biology to be conserved» it should also be possible to transfer the knowledge gained from our studies lo other species. Therefore, this proposal also is of general importance for the fields of regenerative medicine, cancer biology, and aging.

甲虫 Tribolium 代表端营养卵巢，与果蝇的多营养卵巢有根本不同。为了深入了解端营养卵巢器官发生、卵子发生和体细胞干细胞生物学的分子机制，我们建议通过 RNAi 对果蝇中的所有 Tribolium 基因进行功能分析。生殖干细胞和成体干细胞在空间和时间上的紧密关联使得分析成体干细胞谱系非常困难。然而，蛹期的系统性 RNAi 可以对体细胞谱系进行功能分析，而不受生殖系发育干扰，另一方面，幼虫 RNAi 可以分析这些早期过程，例如卵巢器官发生和生殖系衍生细胞的规格。拟议的项目将增强我们对不同卵巢类型的发育和进化的了解，此外，通过 iBeetle 筛选，我们将首次确定卵巢发育和体细胞所需的一套全面的基因。干细胞生物学，这在果蝇中尚不可能实现，因为我们期望控制干细胞生物学的主要机制得以保留，因此也应该可以将我们的研究中获得的知识转移到其他物种。对于再生医学、癌症生物学和衰老领域具有普遍重要性。