RUI MRI Genomic and Genetic Analysis Research in Molecular Biology at Pomona College

RUI 波莫纳学院分子生物学 MRI 基因组和遗传分析研究

• 批准号: 0318944
• 负责人: Laura Hoopes
• 金额: $ 23.58万
• 依托单位: Pomona College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0318944&HistoricalAwards=false
• 关键词: RUI; MRI; Genomic; Genetic; Analysis

A grant has been awarded to Pomona College under the direction of Dr. Laura Hoopes to facilitate genomic and genetic analysis research. The grant will provide funds for the acquisition of a microarray scanner, associated computers and software, a phosphoimager, and a sequence detection system for real-time PCR. All of these instruments will be used in faculty-student collaborative research in 5 different laboratories at Pomona College. The microarray scanner will be used to examine gene expression changes in yeast aging, in mutants affecting the ability of yeast to recombine by recognizing short sequences of similar DNA, and in archaebacteria that grow in hot vents on the ocean floor. In addition, the microarray scanner will be used in projects to look for changes in gene expression in strains with a homing endonuclease (and mutant versions of it) cloned into the bacterium Escherichia coli. It will also be used to examine gene expression in developmental mutants of Drosophila that disrupt transport of membrane-enclosed vesicles within cells. Real-time PCR will be used to quantitate the results of microarray analyses, and to examine plasmids for double strand break (DSB) repair. The phosphoimager will be used in faculty-student research to examine the creation and repair of DSBs, and in ChIP assays. The phosphoimager will also be used to quantitate the amounts of various DNA replication structures that can be detected using 2-dimensional gel electrophoresis, allowing us to analyze replication fork stalling in aged vs. mutant cells.There are a number of ways this grant will impact the scientific infrastructure. Many students at Pomona College will gain experience in cutting-edge genetics and genomics research, thus reinforcing their interest in pursuing careers in these fields. The microarray scanner and phosphoimager will facilitate a major experiment in the Genetic Regulation in Eukaryotes and other research-style classes at Pomona College, where students will learn the context of these techniques, as well as apply their knowledge to a real problem in the laboratory. Finally, the microarray scanner will enable scanning for the Genome Consortium for Active Teaching (GCAT) West Coast members, whose undergraduate student research projects and research-oriented courses impact more than 100 students annually.

在劳拉·霍普斯（Laura Hoopes）博士的指导下，已向波莫纳学院（Pomona College）授予了赠款，以促进基因组和遗传分析研究。 该赠款将为获取微阵列扫描仪，相关的计算机和软件，磷光iMimager以及实时PCR的序列检测系统提供资金。 所有这些工具都将用于波莫纳学院5个不同实验室的教师合作研究。 微阵列扫描仪将用于检查酵母衰老的基因表达变化，突变体的突变体，通过识别相似DNA的短序列以及在海底的热通风口中生长的大细菌，影响酵母的能力。 此外，微阵列扫描仪将用于项目中，以寻找带有归因核酸内切酶（和突变版本）的菌株中基因表达的变化，并将其克隆到大肠杆菌中。 它也将用于检查果蝇发育突变体中破坏细胞内膜封闭囊泡的转运的基因表达。 实时PCR将用于量化微阵列分析的结果，并检查质粒的双链断裂（DSB）修复。 磷酸iM剂将用于教师研究中，以检查DSB的创建和修复以及CHIP分析。 磷酸iMimager还将用于量化可以使用二维凝胶电泳检测到的各种DNA复制结构的量，使我们能够分析老年与突变细胞中停滞不前的复制叉。这有很多方法会影响科学基础结构。 波莫纳学院（Pomona College）的许多学生将获得尖端遗传学和基因组学研究的经验，从而增强他们对从事这些领域的职业的兴趣。 微阵列扫描仪和磷酸imim剂将促进Pomona College的真核生物和其他研究风格的遗传调节的重大实验，在那里学生将学习这些技术的背景，并将其知识应用于实验室中的真正问题。 最后，微阵列扫描仪将对西海岸的活跃教学（GCAT）成员进行扫描，其本科生研究项目和面向研究的课程每年影响100多名学生。