Biochemistry of the Anaerobic Dehalogenation of Chlorinated Aromatics

氯化芳烃厌氧脱卤的生物化学

• 批准号: 0211730
• 负责人: Stephen Ragsdale
• 金额: $ 43万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-10-01 至 2006-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0211730&HistoricalAwards=false
• 关键词: Biochemistry; Anaerobic; Dehalogenation; Chlorinated; Aromatics

This study looks at a genus of microbes, Desulfitobacteria, that can detoxify halogenated organic pollutants such as polychlorinated biphenyls (PCBs). PCBs are listed as a "dirty dozen" persistent organic pollutant (POP) that, although banned, remain in the environment and cause lasting damage to animal health and the environment. The long-term goal is to help achieve the aim of a 2001 United Nations treaty to phase out and eventually eliminate these POPs. Thus a better understanding of how to accelerate the natural biodegradation of such compounds would be of significant value to society. Through a process known as dehalorespiration, these microbes biodegrade halogenated compounds while gaining metabolic energy. When Desulfitobacteria sense the presence of a halogenated compound, they produce a host of proteins that enable them to remove the chlorine substituent, which is the first step in its detoxification. A manifold of techniques are used to study dehalorespiration, including enzymology, molecular biology and genetics, spectroscopy, and electrochemistry. The first objective of this project is to elucidate the catalytic mechanism of the dehalogenase enzyme, which removes the chlorine substituent. To achieve this aim, each of the steps in the dehalogenase catalytic cycle will be elucidated and the role of the metal cofactors (vitamin B12 and the iron-sulfur clusters) will be determined. The second objective is to study the transcriptional regulatory protein (CprK) that controls when and how much of the dehalorespiration proteins are produced. When CprK binds dilute solutions of the halogenated compound, it attaches to a specific DNA regulatory sequence and accelerates the rate of production of the components of the dehalorespiration system. Interactions between the transcriptional regulatory protein (CprK) and the xenobiotic and between CprK and the DNA sequence will be studied. This project also includes plans to promoting teaching, training and learning; to enhance the University of Nebraska research infrastructure; and to broadly disseminate the research results.

这项研究着眼于一种微生物属脱硫二核细菌的属，该属可以对卤代有机污染物（如多氯化双苯基（PCB））排毒。 PCB被列为“肮脏”持久性有机污染物（POP），尽管被禁止，但仍留在环境中，并对动物健康和环境造成持久损害。 长期目标是帮助实现2001年联合国条约的目标，以逐步淘汰并最终消除这些流行音乐。 因此，更好地理解如何加速这种化合物的自然生物降解对社会具有重要的价值。 通过称为脱甲刺的过程，这些微生物在获得代谢能量的同时生物降解化合物。 当脱硫细菌感觉到卤化化合物的存在时，它们会产生许多蛋白质，使它们能够去除氯取代基，这是其排毒的第一步。 一系列技术用于研究脱氧吸期，包括酶学，分子生物学和遗传学，光谱和电化学。 该项目的第一个目的是阐明去除氯化物酶的脱核酶的催化机理。 为了实现这一目标，将阐明脱核酶催化循环中的每个步骤，并确定金属辅因子（维生素B12和铁硫簇）的作用。 第二个目标是研究控制何时何时以及产生多少脱氧蛋白的转录调节蛋白（CPRK）。 当CPRK结合卤化化合物的稀释溶液时，它将附着在特定的DNA调节序列上，并加速了脱甲孔刺激系统的成分的生产率。转录调节蛋白（CPRK）与异种生物之间以及CPRK与DNA序列之间的相互作用将进行研究。 该项目还包括促进教学，培训和学习的计划；增强内布拉斯加州大学研究基础设施；并广泛传播研究结果。