Collaborative Research: The Green Plant BAC Library Project

合作研究：绿色植物BAC图书馆项目

• 批准号: 0207202
• 负责人: Claude dePamphilis
• 金额: $ 7.18万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0207202&HistoricalAwards=false
• 关键词: Collaborative; Research; Green; Plant; BAC

0207202de PamphillisThis research project is designed to accomplish two aims: Aim 1: To construct and array high-quality BAC libraries to provide a genomic resource on a wide range of species. Aim 2: To enable researchers working with green algae, non-seed land plants, and seed plants (including flowering plants) to identify genes critical for understanding plant form and function and how land plants arose and diversified. This project will enable progress toward an understanding of the genetic basis for the transitions that mark the most fundamentally important steps in green plant evolution. The Deep Green community (http://ucjeps.herb.berkeley.edu/bryolab/deepgene/index.html) will help to provide an infrastructure for ongoing scientific exchange. Bioinformatics and a web site will be provided to the community to access these resources (http://www.genome.clemson.edu/). Selected Species: Desired coverage is given in parentheses. Where two coverage values are listed, two libraries using different restriction enzymes will be made.Green algae: Volvox carteri (5x, 5x) Caulerpa mexicana (8x)Mesostigma viride (8x)Coleochaete orbicularus (6.4x, 6.4x)Chara aspera (6x)Non-seed plants: Marchantia polymorpha (8x)Anthoceros sp. (6.3x, 6.3x)Lycopodium lucidulum (5.1x, 5.1x)Angiopteris erecta (6.2x, 6.2x)Ceratopteris richardii (4.8x, 4.8x)Marsilea quadrifolia (5.6x, 5.6x) Seed plants:Amborella trichopoda (5.5x)Nuphar adventa (7x)Acorus gramineus (7x)Lirodendron tulipifera (7x)Mimulus guttatus (7x)

0207202de Pamphillis 该研究项目旨在实现两个目标： 目标 1：构建和排列高质量的 BAC 文库，以提供广泛物种的基因组资源。 目标 2：使研究人员能够研究绿藻、非种子陆地植物和种子植物（包括开花植物），以确定对于理解植物形态和功能以及陆地植物如何产生和多样化至关重要的基因。 该项目将有助于理解转变的遗传基础，这些转变标志着绿色植物进化中最根本的重要步骤。 Deep Green 社区 (http://ucjeps.herb.berkeley.edu/bryolab/deepgene/index.html) 将帮助为持续的科学交流提供基础设施。 将向社区提供生物信息学和网站以访问这些资源（http://www.genome.clemson.edu/）。 选定的物种：所需的覆盖范围在括号中给出。 如果列出两个覆盖值，则将建立两个使用不同限制性内切酶的文库。 绿藻：Volvox carteri (5x, 5x) Caulerpa mexicana (8x)Mesostigma viride (8x)Coleochaete orbillaryus (6.4x, 6.4x)Chara aspera (6x) )非种子植物：地钱 (8x)Anthoceros sp. (6.3x, 6.3x)Lycopodium lucidulum (5.1x, 5.1x)Angiopteris直立 (6.2x, 6.2x)Ceratopteris richardii (4.8x, 4.8x)Marsilea fourfolia (5.6x, 5.6x) 种子植物：Amborella trichopoda (5.5 x)独孤草(7x)石菖蒲 (7x)鹅掌楸 (7x)酸浆 (7x)