Novel Optical Biosensors for Hazardous Ions

用于有害离子的新型光学生物传感器

• 批准号: 0097442
• 负责人: Richard Thompson
• 金额: $ 41.81万
• 依托单位: University of Maryland at Baltimore
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0097442&HistoricalAwards=false
• 关键词: Novel; Optical; Biosensors; Hazardous; Ions

0097442ThompsonThe objective of this research is the development of new fluorescence-based sensors with high sensitivity and accuracy for important industrial analytes for which current chemical sensors are inadequate or nonexistent. In earlier work the investigators demonstrated that carbonic anhydrase-based biosensors were capable of transducing the levels of certain transition metals as changes in fluorescence intensity, lifetime, and anisotropy. These sensors were able to rapidly and continuously determine transition metal ions such copper and zinc at sub-part per billion levels in the presence of calcium and magnesium at million-fold higher concentrations. In this research the metal ion approach will be extended for the detection of the heavy metals lead, mercury and cadmium. The bulk of the engineering effort in this phase of the research will occur at the molecular level, in the construction of the fluorescent transducer/recognition molecules and determination of their properties. In addition, a previously developed approach, which uses fluorescence lifetime to detect anions such as cyanide and cyanate, will be adapted for the determination of the anions chromate, arsenate, and nitrate.

0097442THOMPSONPONS这项研究的目标是开发新的基于荧光的传感器，对于当前化学传感器不足或不存在的重要工业分析物​​具有高灵敏度和准确性。 在较早的工作中，研究人员表明，基于碳酸酐酶的生物传感器能够随着荧光强度，寿命和各向异性的变化而能够转换某些过渡金属的水平。 这些传感器能够快速，连续地确定过渡金属离子，例如在钙和镁的存在下，铜和锌的浓度高于百万倍。 在这项研究中，将扩展金属离子方法以检测重金属铅，汞和镉。 在这一研究阶段的大部分工程工作将在分子水平上发生，在荧光换能器/识别分子的构建中及其性能的测定。 此外，将使用荧光寿命检测氰化物和氰酸盐等阴离子的一种先前开发的方法，以确定阴离子铬酸盐，砷酸盐和硝酸盐。