Molecular analysis of two ethylene-induced cell death processes in rice (Oryza sativa L.)

水稻 (Oryza sativa L.) 中两种乙烯诱导的细胞死亡过程的分子分析

• 批准号: 143567865
• 负责人: Privatdozentin Dr. Bianka Steffens
• 金额: --
• 依托单位: Zentrum für Molekularbiologie der Pflanzen (ZMBP)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/143567865?language=en
• 关键词: Molecular; analysis; two; ethylene; induced

Cell death is an intrinsic part of plant development. It can be developmentally regulated or be induced by environmental signals. Flooded plants suffer from lack of oxygen. To avoid hypoxic stress, well adapted plants such as rice improve their oxygen supply through growth of roots at the stem and through formation of internal air spaces (aerenchyma). Growth of roots is preceded by death of epidermal cells that cover the root primordia. Epidermal cell death as well as aerenchyma formation are regulated by the plant hormone ethylene. We previously identified morphological and molecular differences between epidermal cells that undergo cell death and those that do not. In the current project we attempt to define the cellular characteristics and the signaling pathway of epidermal cell death. We will functionally characterize genes that were found to be differentially active in dying versus non-dying cells with a focus on genes which determine cell identity. Such genes may be responsible for the ability to undergo cell death and for the local confinement of cell death. With the help of mutants, genes that have been recognized as being important for epidermal cell death will be studied in aerenchyma formation, which represents a second ethylene-induced local cell death process. This comparative part of the study should help pinpoint common and diverging pathways in the signaling and execution of cell death by ethylene.

细胞死亡是植物发育的固有部分。它可以在发育过程中受到调节或由环境信号诱导。被水淹的植物会缺氧。为了避免缺氧应激，适应性强的植物（例如水稻）通过茎根的生长和内部空气空间（通气组织）的形成来改善氧气供应。根生长之前覆盖根原基的表皮细胞死亡。表皮细胞死亡以及通气组织的形成受植物激素乙烯的调节。我们之前发现了经历细胞死亡和未经历细胞死亡的表皮细胞之间的形态和分子差异。在当前的项目中，我们试图定义表皮细胞死亡的细胞特征和信号通路。我们将对在死亡细胞和非死亡细胞中被发现具有差异活性的基因进行功能表征，重点关注决定细胞身份的基因。这些基因可能负责细胞死亡的能力和细胞死亡的局部限制。在突变体的帮助下，将在通气组织形成中研究已被认为对表皮细胞死亡重要的基因，这代表了第二个乙烯诱导的局部细胞死亡过程。该研究的比较部分应有助于查明乙烯信号传导和执行细胞死亡的常见和不同途径。