Comprehensive Upgrade of a Shared Campus Nuclear Magnetic Resonance Facility

校园共享核磁共振设施全面升级

• 批准号: 0079461
• 负责人: Gary Smith
• 金额: $ 15.08万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0079461&HistoricalAwards=false
• 关键词: Comprehensive; Upgrade; Shared; Campus; Nuclear

This award will fund a cryoprobe for state-of-the-art nuclear magnetic resonance (NMR) studies of biological macromolecules and natural products. The cryoprobe will be used at the UC Davis NMR Facility by researchers in the fields of chemistry, biochemistry, and structural biology. Specific examples include studies of the molecular structure and dynamics of proteins, nucleic acids, carbohydrates, lipids, and natural products from a variety of sources. The high sensitivity cryoprobe dramatically increases experimental sensitivity, reduces experimental run times, and lowers the minimum amount of sample needed for study, thus opening new areas of productive research. The cryoprobe package consists of a cryogenically cooled NMR probe, a mounting platform, associated cryogenic pumps and tubing, and a cryogenically cooled preamplifier. The probe will be a triple resonance type, with proton observation and both carbon and nitrogen decoupling channels. A Z-field gradient coil is also part of the probe. The probe sensitivity is approximately two to four times that of non-cryogenic probes for the same sample volume and concentration. This increase translates into reductions in run time of approximately four to sixteen fold. Such a drastic savings in run time effectively provides another entire spectrometer at a fraction of the cost. The probe will be capable of multi-resonance, multi-dimensional solution studies of biological macromolecules and natural products. The instrumentation will become part of the UCD NMR Facility. The Facility is utilized annually by over 40 faculty members (in approximately 20 departments of the Davis campus). These research groups have approximately 140 students and 50 postdoctoral fellows, of whom approximately 40 individuals are regular hands-on users of the instrumentation. The NMR spectrometers are an ancillary part of 14 classes annually with an average total enrollment of over 100 students. The spectrometers also serve as a state of the art resource for student and faculty recruitment. The cryoprobe will thus make a significant contribution to the research and educational infrastructure at the University of California, Davis.

该奖项将为生物大分子和天然产物的最先进的核磁共振（NMR）研究提供冷冻探针。 该冷冻探针将由化学，生物化学和结构生物学领域的研究人员在UC Davis NMR UC NMR设施中使用。 具体的例子包括对蛋白质的分子结构和动力学的研究，核酸，碳水化合物，脂质和来自多种来源的天然产物。 高灵敏度的冷冻探针大大提高了实验敏感性，减少了实验性运行时间，并降低了研究所需的最低样本量，从而打开了新的生产性研究领域。 冷冻探针包由低温冷却的NMR探针，一个安装平台，相关的低温泵和管道以及低温冷却的前置放大器。 该探针将是一种三重共振类型，具有质子观测以及碳和氮解耦通道。 Z场梯度线圈也是探针的一部分。 探针敏感性约为相同样品体积和浓度的非晶探针的两到四倍。 这种增加的运行时间约为四到十六倍。 在运行时间中，如此巨大的节省有效地提供了另一个整个光谱仪的成本。该探针将能够对生物大分子和天然产物进行多谐，多维溶液研究。 该仪器将成为UCD NMR设施的一部分。 该设施每年由40多名教职员工（在戴维斯校园的大约20个部门）使用。 这些研究小组有大约140名学生和50名博士后研究员，其中大约40个人是该乐器的定期动手使用者。 NMR光谱仪是每年14个课程的辅助部分，平均总入学人数为100多名学生。 这些光谱仪还可以作为学生和教师招聘的最先进的资源。因此，该冷冻探针将为加利福尼亚大学戴维斯分校的研究和教育基础设施做出重大贡献。