RUI: Collaborative Proposal: Biochemical and Physiological Adaptation to Temperature in a Montane Insect Herbivore

RUI：合作提案：山地昆虫草食动物对温度的生化和生理适应

• 批准号: 0078464
• 负责人: Elizabeth Dahlhoff
• 金额: $ 20.34万
• 依托单位: Santa Clara University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0078464&HistoricalAwards=false
• 关键词: RUI; Collaborative; Proposal; Biochemical; Physiological

This research investigates evolution in high elevation populations of the leaf beetle Chrysomela aeneicollis. Populations in the Sierra Nevada (California) are on the southern edge of the species' range. Previous studies found that drainages differ in air temperature and beetle populations fluctuate with changes in climate. Frequencies of different forms (genotypes) of the temperature-sensitive enzyme phosphoglucose isomerase (PGI) vary among drainages, suggesting that natural selection acts on PGI. Heat shock protein (Hsp) levels in beetles correspond to air temperature differences and depend on PGI genotype. The proposed research will investigate the relationship between temperature and functional property of PGI genotypes (i.e. their ability to catalyze biochemical reactions fundamental to metabolism). Second, the effects of temperature on the physiology of beetles of different PGI genotype will be studied, using Hsp's and ubiquitin conjugates as indices of physiological stress. Third, survival and reproduction of beetles of different PGI genotype in nature will be compared. The research will provide important information about the evolutionary significance of enzyme polymorphisms. It may add insight about the impact of climate change on native insects. Finally, it will allow undergraduates to participate in basic research, enhance their educational experience and expand their opportunities for the future

这项研究调查了叶甲虫Chrysomela aeneicollis的高升高种群的进化。 内华达山脉（加利福尼亚州）的人口处于该物种范围的南部边缘。 先前的研究发现，空气温度和甲虫种群随着气候变化而波动。 温度敏感酶磷酸葡萄糖异构酶（PGI）的不同形式（基因型）的频率在排水之间有所不同，这表明自然选择作用于PGI。 甲虫中的热休克蛋白（HSP）水平对应于空气温度差异，并取决于PGI基因型。 拟议的研究将研究PGI基因型的温度与功能特性之间的关系（即它们催化代谢基础的生化反应的能力）。 其次，将研究温度对不同PGI基因型甲虫生理的影响，使用HSP和泛素结合物作为生理胁迫的指标。 第三，将比较自然界中不同PGI基因型甲虫的生存和繁殖。 该研究将提供有关酶多态性进化意义的重要信息。 它可能会增加有关气候变化对本地昆虫的影响的见解。 最后，它将允许本科生参与基础研究，增强他们的教育经验并扩大未来的机会