Molecular Genetic Characterization of Pathways Involved in Establishing Abaxial Cell Fate in Arabidopsis Carpels

拟南芥心皮中参与建立远轴细胞命运的途径的分子遗传学特征

• 批准号: 9986054
• 负责人: John Bowman
• 金额: $ 36万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9986054&HistoricalAwards=false
• 关键词: Molecular; Genetic; Characterization; Pathways; Involved

Bowman9986054While significant progress has been made in the understanding of the molecular mechanisms by which organ identity is specified in developing flowers, much less is known about the mechanisms of cell fate specification within individual organs. A molecular genetic approach was chosen to elucidate how cell fate is specified in the carpel, initially focusing on the role of CRABS CLAW. It has recently been shown that CRABS CLAW is required for the specification of abaxial cell fate in the carpels. This conclusion was based on genetic analyses of both loss- and gain-of-function alleles of CRABS CLAW as well as comparisons with closely related gene family members.The development of adaxial tissues in abaxial positions in the carpel requires twoconditions: first, the loss of abaxial cell fate, and second, the loss of suppression of genes promoting meristematic growth. The second condition must be met in order to allow the development of placental meristems on the abaxial surfaces of the carpel. This proposal is focused on two genes, CRABS CLAW and KANADI, that appear to act independently in a partially redundant manner to promote the specification of abaxial cell fate in the medial regions of the carpel. While single mutants in either gene do not display a loss of polar differentiation in the carpel, in crabs claw kanadi double mutants mirror image duplications of adaxial tissues develop in abaxial positions of the carpel. The overall aims of this proposal are to identify and characterize genes andphytohormones that play a role in either specification of abaxial cell fate or suppression and/or activation of meristematic potential

Bowman9986054在理解在花朵中指定器官身份的分子机制方面取得了重大进展，对单个器官内细胞命运规范的机制知之甚少。选择了一种分子遗传学方法来阐明在地球上如何指定细胞命运，最初着重于蟹爪的作用。最近已经显示，螃蟹爪是按照地表中肥细胞命运的规格所必需的。该结论基于对螃蟹爪的损失和功能获得等位基因的遗传分析，以及与密切相关的基因家族成员的比较。在地球中舒缓地位的轴向组织的发展需要两次条件：首先，首先，阿比亚细胞命运的损失，第二，促进基因抑制的丧失，促进基因的抑制作用。必须满足第二种条件，以便允许在地球的不稳定表面上发展胎盘分生物。该提案的重点是两个基因，即蟹爪和卡纳迪，它们似乎以部分冗余的方式独立起作用，以促进皮肤中间区域中巴细胞命运的规格。虽然这两个基因中的单个突变体都不显示地球上极性分化的损失，但在螃蟹爪卡纳迪双重突变体中，镜像的近距离组织的图像复制在地球的弱点位置出现。该提案的总体目的是识别和表征基因和肌激素，这些基因和肾上腺激素在Abaxial细胞命运或抑制和/或分生势的抑制和/或激活中发挥作用