SGER: Crystallization of a P1-Type Heavy Metal Transport ATPase

SGER：P1 型重金属转运 ATP 酶的结晶

• 批准号: 9985771
• 负责人: Jose Arguello
• 金额: $ 9.96万
• 依托单位: Worcester Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9985771&HistoricalAwards=false
• 关键词: SGER; Crystallization; P1; Type; Heavy

9985771ArguelloIons are transported across membranes of living organisms using metabolic energy derived from ATP. The transport proteins are enzymes that are imbedded in the membrane and classified as P-type ATPases. There is not a complete understanding of the processes performed by these enzymes due in part to a lack of knowledge of their atomic structures. During the last few years, a new group of P-type ATPases (P1-type) has been identified in prokaryotes that transport heavy metals such as Cu+1, Cu+2, Zn+2, Cd+2, and others into cells. These enzymes have the same mechanistic characteristics as other P-type ATPases, but they are smaller in size. This characteristic makes them easier to isolate, purify, crystallize and obtain their atomic structure. The detailed structural information obtained through crystallographic analysis of this sub-class of P-type ATPases will provide information on the ion selectivity and energy transduction mechanisms of other P-type ATPases and a clearer understanding of energy coupled ion transport. This is especially important since this knowledge may also provide a basis for the engineering of microorganisms that can be used effectively in bioremediation.

9985771arguelloions使用来自ATP的代谢能量在生物体的膜上运输。 转运蛋白是嵌入膜中并归类为P型ATPases的酶。 对于这些酶执行的过程，部分原因是由于缺乏对原子结构的了解，因此没有完全了解这些酶的过程。 在过去的几年中，在将重金属（例如Cu+1，Cu+2，Zn+2，Cd+2等）运输到细胞的原核生物中已经确定了一组新的P型ATPase（P1型）（P1型）。这些酶具有与其他P型ATPases相同的机械特性，但大小较小。 这种特征使它们更容易隔离，纯化，结晶并获得其原子结构。 通过对P型ATPASE的晶体学分析获得的详细结构信息将提供有关其他P型ATPases的离子选择性和能量转导机制的信息，以及对能量耦合离子传输的更清晰的了解。 这一点尤其重要，因为这些知识也可能为可以在生物修复中有效使用的微生物的工程提供基础。