CAREER: Understanding the Role of Matrix in the Cryopreservation of Liver Systems

职业：了解基质在肝脏系统冷冻保存中的作用

• 批准号: 9984648
• 负责人: Robin Coger
• 金额: $ 20万
• 依托单位: University of North Carolina at Charlotte
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-15 至 2005-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9984648&HistoricalAwards=false
• 关键词: CAREER; Understanding; Role; Matrix; Cryopreservation

9984648CogerThe research program of this career award seeks to advance our ability to safely preserve and store liver replacement devices at low temperature such that a inventory of viable hepatocytes is readily available when the need for liver support arises. Cryopreservation technology -- or the low term storage of cells, tissue, and organs -- has permitted a variety of cells and tissues to be safely stored at subzero temperatures for future use, yet has not be reported for liver replacement devices. Development of appropriate cryopreservation protocols for these devices requires a understanding of the mechanisms by which cell survival can be enhanced after exposure to low temperatures. The specific aims of this work are to: (1) quantify key physiochemical properties important to the successful preservation of a hepatocyte/collagen system, as a function of temperature; (2) investigate the influence of matrix protein density on hepatocyte survival after cryopreservation; and (3) computationally predict the effects of configuration, volume, and time on the survival of hepatocytes during cryopreservation of hepatic-collagen constructs.The educational component of this award links the research training the students receive with coursework developed expressly to encourage effective interdisciplinary and team interactions. Education through these complementary avenues will aid the students in becoming the developers of future industry and research innovations. The three educational objectives of this award contribute to this goal through new course development, training in interdisciplinary teamwork and problem solving, and mentorship.

9984648Coger 该职业奖的研究计划旨在提高我们在低温下安全保存和储存肝脏替代装置的能力，以便在需要肝脏支持时可以随时获得活肝细胞的库存。 低温保存技术——或者说细胞、组织和器官的短期储存——已经允许将各种细胞和组织安全地储存在零下温度下以供将来使用，但尚未有关于肝脏替代装置的报道。 为这些设备制定适当的冷冻保存方案需要了解暴露于低温后增强细胞存活的机制。 这项工作的具体目标是：（1）量化对于成功保存肝细胞/胶原系统重要的关键理化特性，作为温度的函数； (2)考察基质蛋白密度对冻存后肝细胞存活的影响； (3) 通过计算预测肝胶原结构冷冻保存过程中结构、体积和时间对肝细胞存活的影响。该奖项的教育部分将学生接受的研究培训与专门开发的课程联系起来，以鼓励有效的跨学科和团队互动。 通过这些互补途径进行的教育将帮助学生成为未来工业和研究创新的开发者。该奖项的三个教育目标通过新课程开发、跨学科团队合作和问题解决培训以及指导来实现这一目标。