Acquisition of a Fluorescence-Activated Cell Sorter (FACS) for Research in Biology

采购用于生物学研究的荧光激活细胞分选仪 (FACS)

• 批准号: 9871000
• 负责人: Keith Weaver
• 金额: $ 26.75万
• 依托单位: University of South Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9871000&HistoricalAwards=false
• 关键词: Acquisition; Fluorescence; Activated; Cell; Sorter

This proposal requests funds for a fluorescence- activated cell sorter (FACS) system. A FACS uses laser technology to examine fluorescently tagged intracellular and cell surface proteins and DNA content of live or fixed individual cells and to then sort cells according to their fluorescence and/or DNA content. Research that will be supported by the instrument includes: the action of the molecular chaperonin calreticulin; lentivirus-host interactions; mycoplasm host cell receptors; viral transmission mechanisms; dessication resistance in plants; protein folding by the ER; and hormonal regulation of protein phosphorylation in the ovary.

该提案要求为荧光激活细胞分选仪（FACS）系统提供资金。 FACS 使用激光技术检查荧光标记的细胞内和细胞表面蛋白以及活细胞或固定单个细胞的 DNA 含量，然后根据细胞的荧光和/或 DNA 含量对细胞进行分类。该仪器支持的研究包括：分子伴侣钙网蛋白的作用；慢病毒-宿主相互作用；支原体宿主细胞受体；病毒传播机制；植物的抗旱性； 内质网的蛋白质折叠；以及卵巢中蛋白质磷酸化的激素调节。