SGER: Early Detection of Human Bacterial Pathogens Using Electronic Nose Technology

SGER：利用电子鼻技术早期检测人类细菌病原体

• 批准号: 9807617
• 负责人: H. Troy Nagle
• 金额: $ 10万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-15 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9807617&HistoricalAwards=false
• 关键词: SGER; Early; Detection; Human; Bacterial

9807617 Nagle The investigation will methodically explore the potential for applying emerging "electronic nose" technologies to the rapid detection and analysis of a wide variety of bacterial pathogens. Rapid identification of disease-causing bacterial pathogens would significantly reduce the time required for the diagnostician to prescribe a specific antibiotic. The researchers seek to answer three questions during the twelve months term of their work: 1) Can existing gas sensors detect a wide range of human bacterial pathogens? 2) Can existing gas sensors be used for early identification of one or more human pathogens in culture? 3) Can the sensitivity of conducting polymer sensors be improved for this application by precisely controlling the geometry of the active sensing material? Gas sensor arrays of metal oxide, conducting polymers, and piezoelectric elements are to be assembled in the Biomedical Instrumentation and Biomedical Microsensors Laboratories at NC State University. These sensor arrays will be incorporated into an electronic nose instrument that has been fabricated at NC State. The NC State device and a commercial electronic nose instrument (the AromaScan A32S from the Duke University Taste and Smell Lab) will be used to sample the headspace of bacterial cultures in the Clinical Research Unit #2 at Duke University Medical Center. The sensor data will then be analyzed using normalization, feature extraction, and pattern classification algorithms at NC State University. The performance of the sensor arrays and data processing algorithms in differentiating cultured bacteria strains will be evaluated. Sensors will be tested to determine their abilities to detect at least four of ten important drug-resistant microbes. The results of these preliminary laboratory studies are expected to guide future efforts to fabricate and test a hand-held prototype instrument designed for rapid diagnosis of bacterial cultures. This exploratory investigation is believed to be the first research project to make a comprehensive and methodical investigation of the value of the underlying sensor technologies. Success from the investigation could lead to physicians prescribing a disease-specific antibiotic rather than using a broad-spectrum antibiotic until the offending pathogen is identified. Consequently, it is believed the risk will be lowered of the bacteria developing drug resistance. ***

9807617 NAGLE调查将有条不紊地探索将新兴的“电子鼻”技术应用于快速检测和分析多种细菌病原体的潜力。 快速鉴定引起疾病的细菌病原体将显着减少开处方特定抗生素所需的时间。研究人员试图在工作的十二个月期间回答三个问题：1）现有的气体传感器可以检测到广泛的人类细菌病原体吗？ 2）现有的气体传感器是否可以用于培养中一种或多种人类病原体的早期鉴定？ 3）可以通过精确控制活性传感材料的几何形状来提高导电聚合物传感器的灵敏度？ 金属氧化物，导电聚合物和压电元件的气体传感器阵列应在NC州立大学的生物医学仪器和生物医学微传感器实验室中组装。 这些传感器阵列将被合并到已在NC状态制造的电子鼻仪器中。 NC状态设备和商业电子鼻仪器（来自杜克大学的口味和气味实验室的Aromascan A32）将用于在杜克大学医学中心的2临床研究单元中品尝细菌培养的顶空。 然后，将使用NC州立大学的标准化，特征提取和模式分类算法对传感器数据进行分析。 将评估传感器阵列和数据处理算法在分化培养细菌菌株中的性能。将对传感器进行测试，以确定其能力检测十个重要的抗药性微生物中的四个。 这些初步实验室研究的结果有望指导未来的努力，以制造和测试用于快速诊断细菌培养物的手持原型仪器。 探索性研究被认为是对基础传感器技术价值进行全面和有条理的研究项目的第一个研究项目。 调查的成功可能会导致医生开处方疾病特异性抗生素，而不是使用广谱抗生素，直到确定危险的病原体为止。因此，据认为，这种风险将降低产生耐药性的细菌。 ***