Biologische Funktion von Duftstoff-Bindeproteinen, deren Beziehung zu Duftstoffrezeptoren und zur metamorphischen Plastizität der zentralen olfaktorischen Bahn

气味结合蛋白的生物学功能、它们与气味受体的关系以及与中枢嗅觉通路变态可塑性的关系

• 批准号: 122758429
• 负责人: Professor Dr. Joachim Schachtner
• 金额: --
• 依托单位: Niedersächsisches Ministerium für Wissenschaft und Kultur
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/122758429?language=en
• 关键词: Biologische; Funktion; von; Duftstoff; Bindeproteinen

As a member of the largest insect order and a pest of stored agricultural products, the red flour beetle Tribolium castaneum presents an attractive emerging model organism to study olfaction. With the possibility of transgenic approaches such as directed gene expression, powerful reverse genetics based on systemic RNA-interference, the annotated full genome sequence, and its longevity, Tribolium offers a great system to address both odour recognition and discrimination at the periphery as well as the plasticity of the central olfactory pathway. In this project we combine transgenetic, optogenetic, reverse genetic, electrophysiological, chemical ecological, neurobiochemical and neuroanatomical approaches to study the correlation of odorants to odorant binding proteins (OBP) to odorant receptors (OR). The focus of the project will lie on the biological function of OBPs which is still largely unknown, despite their necessity for olfaction. Especially the interaction of OBPs with ORs will be of key interest. Moreover, the established tools will also be applied to study postmetamorphic plasticity of the central olfactory pathway including the first central odour processing centers, the antennal lobes, and higher processing centers, the mushroom bodies.

作为最大昆虫目的成员和储存农产品的害虫，赤拟谷盗为研究嗅觉提供了一种有吸引力的新兴模式生物。凭借定向基因表达、基于系统 RNA 干扰的强大反向遗传学、带注释的全基因组序列及其寿命等转基因方法的可能性，Tribolium 提供了一个出色的系统来解决外围的气味识别和辨别问题，以及中枢嗅觉通路的可塑性。在这个项目中，我们结合转基因、光遗传学、反向遗传学、电生理学、化学生态学、神经生化和神经解剖学方法来研究气味剂与气味剂结合蛋白（OBP）和气味受体（OR）的相关性。该项目的重点将在于 OBP 的生物学功能，尽管它们对于嗅觉是必需的，但其生物学功能仍然很大程度上未知。尤其是 OBP 与 OR 的相互作用将引起人们的主要兴趣。此外，所建立的工具还将应用于研究中央嗅觉通路的变质后可塑性，包括第一个中央气味处理中心，触角叶，和更高级的处理中心，蘑菇体。