SGER: Vector-Transformation System for Frankia

SGER：Frankia 载体转化系统

• 批准号: 9724800
• 负责人: Jerry Johnson
• 金额: $ 5万
• 依托单位: University of Wyoming
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9724800&HistoricalAwards=false
• 关键词: SGER; Vector; Transformation; System; Frankia

Johnson 9724800 Biological nitrogen fixation is a key segment of the nitrogen cycle. The ability to carry out this reaction is limited to a very small number of prokaryotes. A sub-set of these have the capacity to form symbiotic relationships with higher plants to facilitate the process. Bacteria in the genus Rhizobia, gram-negative rods, are the most prominent member of this group of organisms. The genetics and biochemistry of nodulation and nitrogen fixation have been intensively investigated in Rhizobia. This is largely because of the plethora of genetic tools applicable to this group of organisms as well as their relationships with many important crop plants. Frankia are the other major bacterial contributor to symbiotic nitrogen fixation. These organisms are gram positive, filamentous bacteria that nodulate a wide variety of woody plants and shrubs. Superficially, the two types of bacteria have similar modes of operation. However, there are numerous fundamental differences which have been identified between the two systems. Because the Frankia are very slow growing and do not form symbioses with commercially important plants, our knowledge of their genetics and biochemistry is extremely meager. The lack of a vector-transformation system for these bacteria has seriously hampered attempts to understand their molecular biology. Now, the availability of Frankia promoters and the development of electroporation technology as well as highly sensitive, fluorescent DNA and protein probes makes feasible a systematic analysis of the many individual steps in both vector development and transformation that need to be done in order to allow genetic analysis of this important group of ntirogen-fixing bacteria.

约翰逊9724800生物氮固定是氮循环的关键段。 进行此反应的能力仅限于少量的原核生物。 这些子集有能力与较高的植物建立共生关系以促进该过程。 根瘤菌属的细菌，革兰氏阴性棒是这组生物中最杰出的成员。 在根瘤菌中已经对结节和氮固定的遗传学和生物化学进行了深入研究。 这主要是因为适用于这组生物的大量遗传工具及其与许多重要的作物植物的关系。 Frankia是固定共生氮的另一个主要细菌贡献者。 这些生物是革兰氏阳性的，丝状细菌，可以结合各种木本植物和灌木。 从表面上讲，两种类型的细菌具有相似的手术模式。 但是，两个系统之间已经发现了许多基本差异。 由于弗兰克（Frankia）的生长非常缓慢，并且不会与商业重要的植物形成共生，因此我们对它们的遗传学和生物化学的了解非常微不足道。 这些细菌缺乏矢量转化系统已严重阻碍了了解其分子生物学的尝试。 现在，弗兰基亚启动子的可用性以及电穿孔技术的发展以及高度敏感的荧光DNA和蛋白质探针使可行的媒介发展和转化中的许多单个步骤的系统分析，以便对这一重要的ntirogen固定细菌的遗传分析进行遗传分析。