Acquisition of Major Multiuser Research Instrumentation for the Study of Plant, Fungal and Insect Genomics

采购用于植物、真菌和昆虫基因组学研究的主要多用户研究仪器

• 批准号: 9724557
• 负责人: Rod Wing
• 金额: $ 126.5万
• 依托单位: Clemson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 2000-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9724557&HistoricalAwards=false
• 关键词: Acquisition; Major; Multiuser; Research; Instrumentation

The primary objective of this proposal is to acquire major instrumentation for research and training in the analyses of complex plant, fungal and insect genomes. The instrumentation includes: 5 automated DNA sequencers, a robot for preparing DNA sequencing templates, 9 computer workstations for processing sequence and physical mapping data, a robotics system for the development of semi-automated procedures for high through-put screening of thousands of DNA samples for library screening and plant breeding applications, 12 ultra-low freezers for maintenance of large insert B AC libraries, a bank of 6 hybridization incubators and a versatile imaging system for rapid screening and analysis of BAC libraries. The requested instrumentation will be integrated with existing robotics equipment, and ongoing research, service and teaching programs to establish a state-of-the-art Genome Center at Clemson University (CU). The functions of this new CU Genome Center are: 1 ) construction and maintenance of high quality BAC libraries; 2) production and distribution of high-density hybridization filters and DNA pools for library screening and distribution of BAC libraries and clones; 3) positional cloning of agriculturally important genes; 4) physical mapping of whole chromosomes and targeted genomic regions with BAC clones; 5) genome sequencing of important BAC contigs and genomes; 6) application development; and 7) undergraduate, graduate and postdoctoral training. There are three primary activities that would be greatly enhanced by funding this proposal. First, MRI funding would ensure that the CU Genome Center will be capable of serving as a public resource for the construction, maintenance and distribution of large insert BAC libraries of important plant, fungal and insect genomes. Such a centralized resource would provide the research community convenient access to a major tool that is used for positional cloning, physical mapping and genome sequencing. Secondly, MRI funding would allow us to perf orm pilot projects aimed at physical mapping and sequencing of specific genomic regions of genomes that are being targeted for intensive investigation. Successful completion of these projects will put the CU Genome Center in a position to compete effectively for substantial federal support to sequence major portions of genomes such as Arabidopsis, Rice and Neurospora crassa. Lastly, MRI funding will substantially support and enhance undergraduate, graduate, and postdoctoral training in genomics. With access to state-of-the-art instrumentation, the CU-Genome Center will attract students and scholars of high caliber propelling several life sciences programs to national ranking, such as the interdisciplinary program in Genetics. The CU Genome Center, as envisioned above, will be an internationally recognized facility that in turn will produce world class science serving the agricultural genome community at large.

该提案的主要目标是获得用于复杂植物、真菌和昆虫基因组分析的研究和培训的主要仪器。仪器包括：5个自动DNA测序仪、一个用于准备DNA测序模板的机器人、9个用于处理序列和物理图谱数据的计算机工作站、一个用于开发半自动化程序以高通量筛选数千个DNA样本的机器人系统用于文库筛选和植物育种应用，12 个超低温冰箱用于维护大型插入 BAC 文库，一组 6 个杂交培养箱和一个用于快速筛选和分析 BAC 文库的多功能成像系统。 所需的仪器将与现有的机器人设备以及正在进行的研究、服务和教学项目相集成，以在克莱姆森大学 (CU) 建立最先进的基因组中心。这个新的CU基因组中心的功能是：1）高质量BAC文库的建设和维护； 2) 用于文库筛选和分发BAC文库和克隆的高密度杂交过滤器和DNA库的生产和分发； 3）农业重要基因的定位克隆； 4) 用 BAC 克隆对整个染色体和目标基因组区域进行物理定位； 5) 重要BAC重叠群和基因组的基因组测序； 6）应用程序开发； 7）本科生、研究生和博士后培养。 通过资助该提案将大大加强三项主要活动。首先，MRI 资金将确保 CU 基因组中心能够作为公共资源，用于构建、维护和分发重要植物、真菌和昆虫基因组的大型插入 BAC 文库。这种集中的资源将为研究界提供方便地访问用于定位克隆、物理作图和基因组测序的主要工具。其次，MRI 资金将使我们能够执行旨在对正在深入研究的基因组特定基因组区域进行物理绘图和测序的试点项目。这些项目的成功完成将使科罗拉多大学基因组中心能够有效地竞争联邦政府的大力支持，对拟南芥、水稻和粗糙脉孢菌等基因组的主要部分进行测序。最后，MRI 资金将大力支持和加强基因组学方面的本科生、研究生和博士后培训。凭借最先进的仪器，科罗拉多大学基因组中心将吸引高素质的学生和学者，推动多个生命科学项目跻身全国排名，例如遗传学跨学科项目。 如上所述，CU 基因组中心将成为国际公认的设施，进而将产生世界一流的科学成果，为整个农业基因组界服务。