RUI: Genetic and Molecular Polymorphism in Microbotryum Violaceum

RUI：紫罗兰微生物的遗传和分子多态性

• 批准号: 9723623
• 负责人: Raj Sinha
• 金额: $ 31.02万
• 依托单位: Chicago State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9723623&HistoricalAwards=false
• 关键词: RUI; Genetic; Molecular; Polymorphism; Microbotryum

Henry 9723623 This project provides minority students at Chicago State University with opportunities to participate in current research projects at the University of Chicago. The goal is to instruct students in specific areas of genetics to the level that they will be competent to conduct a research project, to guide them to achieve success in their work and to experience the excitement in current genetic biology. Students should come to feel comfortable and confident at a major research university. The experience should stimulate students to consider and to explore careers in the biological sciences. The projects should help students, through their research archievements, to build the necessary self-confidence to enter a graduate degree program. To this end, students will participate in the research on the genetic and molecular evidence of polymorphisms in fungi. The specific organism for this purpose is the anther-smut fungus Microbotryum violaceum. The choice of this species is predicted by ongoing research in the Principal and Co-principal Investigators' laboratories. They have familiarity and long experience with the species. Background work is expected to lead to experiments that challenge students to engage in hands-on and minds-on experience. All projects are selected to provide students with real, rather than contrived, problems. The six projects on transmission gentics focus on testing the hypothesis that the highly mutable loci in M. violaceum may be explained by assuming the activity of a transposible element (TE). Polymorphism in colony color and auxotrophy were selected because the relative ease in experimental manipulation and the accumulation of appropriate collections. Polymorphisms detected by RAPDs is the basis for two projects that may provide molecular markers for the detection of linkage with color, morphology, nutritional requirement and mating type loci. The result of these experiments will also provide information to construct a physical map of the M. vio lacum and other Ustilaginales. It could yield a map of the mitochondrial genome and provide important information on the phylogenetic relatedness between and among the fungi that infect dicot and monocot plants.

Henry 9723623 该项目为芝加哥州立大学的少数族裔学生提供了参与芝加哥大学当前研究项目的机会。 目标是指导遗传学特定领域的学生达到有能力开展研究项目的水平，引导他们在工作中取得成功并体验当前遗传生物学的兴奋。 学生应该在主要研究型大学感到舒适和自信。 这种经历应该激发学生思考和探索生物科学领域的职业。 这些项目应该通过他们的研究成果帮助学生建立进入研究生学位课程所需的自信。 为此，学生将参与真菌多态性的遗传和分子证据的研究。 用于此目的的特定生物体是花药黑粉病真菌 Microbotryum violaceum。 该物种的选择是通过首席和副首席研究人员实验室正在进行的研究预测的。 他们对该物种非常熟悉并拥有长期的经验。 背景工作预计将导致实验，挑战学生参与实践和思维体验。 所有项目的选择都是为了向学生提供真实的问题，而不是人为的问题。 关于传播遗传学的六个项目侧重于检验这样的假设：紫色分枝杆菌中高度可变的基因座可以通过假设转座因子（TE）的活性来解释。 选择菌落颜色多态性和营养缺陷型是因为实验操作相对容易并且积累了适当的集合。 RAPD 检测的多态性是两个项目的基础，这两个项目可以为检测颜色、形态、营养需求和交配型基因座的连锁提供分子标记。 这些实验的结果还将提供构建 M. vio lacum 和其他黑粉菌目物理图谱的信息。 它可以产生线粒体基因组图谱，并提供有关感染双子叶植物和单子叶植物的真菌之间的系统发育相关性的重要信息。