Identification of candidate genes responsible for preformed and inducible defence response in oaks against herbivorous insects

鉴定负责橡树针对草食性昆虫的预形成和诱导防御反应的候选基因

• 批准号: 112762180
• 负责人: Privatdozent Dr. Matthias Fladung
• 金额: --
• 依托单位: Institut für Forstgenetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/112762180?language=en
• 关键词: Identification; candidate; genes; responsible; preformed

The defoliation of oaks is an urgent problem for forestry in Central Europe. During the last outbreak of the green oak leaf roller (Tortrix viridana) in 2003-2005, we observed fundamental differences in the defoliation level of individual Quercus robur trees in Germany. Some of the trees seem to be somehow “tolerant” (T oaks) against the insects grub while some seem to be conspicuously “susceptible” (S oaks). Within this study we aim to identify the underlying molecular and biochemical mechanisms in oaks responsible for the behavioural preference of Tortrix viridana. By means of combined feeding choice experiments and biochemical analysis of preformed and induced defence mechanisms in T and S oak phenotypes, we will identify the metabolic/chemical basis of the observed differences as prerequisite for the selection of candidate genes expressed in tolerant trees, but not in susceptible ones or vice versa after insects feeding. We will identify functional genes involved in the defence response (i.e. phenolic compounds and plant volatiles) of oak (Q. robur) trees via microarray analysis in already identified T and S oak phenotypes in a research area in North Rhine-Westphalia (Western Germany). This approach will give us a deep insight into the functional genomics of Q. robur relating to the feeding of herbivorous insects. With the identification of molecular and biochemical markers of “Tortrix tolerance” in oaks we can contribute to decision support in sustainable forest management.

橡树的脱叶是中欧林业的紧迫问题。在2003年至2005年的绿橡树叶辊（Tortrix viridana）的最后一次爆发中，我们观察到德国单个槲皮树的脱叶水平的基本差异。有些树似乎在某种程度上是“宽容”（t oaks）对昆虫g的，而有些树似乎显然是“易感性的”（s oaks）。在这项研究中，我们旨在确定负责Tortrix Viridana行为偏好的橡木中的基本分子和生化机制。通过对T和S OAK表型中预先形成和诱导的防御机制的结合喂养选择实验以及生化分析，我们将确定观察到的差异的代谢/化学基础，是选择在耐受树中表达的候选基因的先决条件，但在易于捕食昆虫或昆虫后，在耐受树中表达的候选基因。我们将通过在北莱茵 - 韦斯特法里亚（Western Dermany）的研究领域中已经鉴定出的T和S OAK表型中的微阵列分析来鉴定橡木（Q. Robur）树的防御反应（即酚类化合物和植物挥发物）的功能基因。这种方法将使我们深入了解Q. Robur的功能基因组学与食草昆虫的喂养有关。通过鉴定橡木中“ tortrix耐受性”的分子和生化标记，我们可以为可持续森林管理中的决策支持做出贡献。