The role of hydrogen peroxide as signalling molecule in leaf senescence and its impact on senescence-induced nitrogen mobilisation

过氧化氢作为信号分子在叶片衰老中的作用及其对衰老诱导的氮动员的影响

基本信息

批准号：
111000925
负责人：
Professorin Dr. Ulrike Zentgraf
金额：
--
依托单位：
Abteilung für Allgemeine Genetik
依托单位国家：
德国
项目类别：
Research Units
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/111000925?language=en
关键词：
role hydrogen peroxide signalling molecule

项目摘要

Despite the importance of senescence, our knowledge on the regulatory mechanisms of this process is still fragmentary. For the model plant Arabidopsis thaliana we have previously shown that during the onset of leaf senescence the hydrogen peroxide concentration in the leaf cells increases by a complex regulatory interplay of the hydrogen peroxide scavenging enzymes. This hydrogen peroxide peak is involved in the onset of senescence most likely by transcriptional up-regulation of important senescence-associated transcription factors like WRKY53 or NAC92. In oilseed rape, hydrogen peroxide is also up-regulated during developmental senescence. In this approach, we will analyze the role of hydrogen peroxide in the expression of genes involved in the mobilization of nitrogenous compounds in more detail in the crop plant oilseed rape. Here, we will focus on the expression of seed storage protein genes which were unexpectedly expressed in leaves following the hydrogen peroxide profiles and possibly serving as a putative interim N storage. This was detected only in oilseed rape but not in Arabidopsis. In addition, we have several lines of evidence that the cellular compartment where the hydrogen peroxide is formed is important for its signalling function. Therefore, we would like to proceed with the establishment of an in vivo imaging system for hydrogen peroxide using a hydrogen peroxide sensitive GFP directed to different cellular compartments in Arabidopsis and oilseed rape to understand its regulatory role more precisely and in the long run create plants which can be very specifically manipulated in senescence regulation and nitrogen mobilization.Keywords: Hydrogen peroxide as signalling molecule, hydrogen peroxide scavenging enzymes (catalase and ascorbate peroxidase), role of hydrogen peroxide in gene expression of SAGs, in vivo hydrogen peroxide imaging system.

尽管衰老很重要，但我们对这一过程的调节机制的了解仍然是零散的。对于模型植物拟南芥，我们先前已经表明，在叶片衰老开始时，叶片细胞中的过氧化氢浓度通过过氧化氢清除酶的复杂调节相互作用而增加。该过氧化氢峰参与衰老的发作，最有可能是由于重要衰老相关转录因子（如WRKY53或NAC92）的转录上调。在油料强奸中，在发育衰老期间，过氧化氢也被上调。在这种方法中，我们将分析过氧化氢在作物植物油种子强奸中更详细地详细介绍参与氮化合物的基因表达的作用。在这里，我们将重点关注种子储存蛋白基因的表达，这些蛋白基因在过氧化氢剖面后意外表达，并可能用作假定的临时n储存。这仅在油籽强奸中检测到，但在拟南芥中没有检测到。此外，我们有几条证据表明，形成过氧化氢的细胞室对于其信号传导功能很重要。因此，我们希望使用过氧化氢敏感的GFP来建立过氧化氢的体内成像系统，该系统针对拟南芥和油料的强奸中的不同细胞隔室，以更精确地理解其调节作用，从而更加精确地了解其长期的植物，在长期以来可以在杂种和杂种中非常特殊地操纵液化剂。过氧化物清除酶（过氧化酶和抗坏血酸过氧化物酶），过氧化氢在SAGS基因表达中的作用，体内过氧化氢成像系统。