Plant Genes Involved in Agrobacterium-Mediated Transformation

参与农杆菌介导转化的植物基因

基本信息

批准号：
9630779
负责人：
Stanton Gelvin
金额：
$ 36万
依托单位：
Purdue Research Foundation
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1996
资助国家：
美国
起止时间：
1996-08-01 至 1999-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630779&HistoricalAwards=false
关键词：
Plant Genes Involved Agrobacterium Mediated

项目摘要

9630779 Gelvin Agrobacterium tumefaciens causes the disease Crown Gall on many species of plants. The molecular mechanism of Crown Gall tumorigenesis involves the binding of bacterial cells to the plant cell wall, and the processing of a region of the Ti-(tumor inducing) plasmid and transfer of this T-(transfer) DNA to plant cells. Ti plasmid has been modified to be used as a vector for genetic engineering in plants. As a result of two decades of research, we know much about the contribution of bacterial genes and proteins to T-DNA transfer and tumorigenesis. However, we know little about the role plant genes and proteins play in these processes. In an attempt to identify host-encoded factors necessary for Agrobacterium binding, T-DNA transfer and integration, and phytohormone signal transduction, Dr. Gelvin has recently screened different Arabidopsis thaliana ecotypes and T-DNA insertion mutants for susceptibility to crown gall tumorigenesis, as well as the ability to express other T-DNA-encoded functions both transiently and stably. In this research project, Dr. Gelvin's proposes experiments to identify specifically the steps of crown gall tumorigenesis that are blocked in Arabidopsis T-DNA insertion mutants that have already been identified. The characterization of these host genes will not only increase our understanding of crown gall tumorigenesis, but will also contribute to our knowledge of basic plant cell biology. Finally, an understanding and the isolation of the host genes involved in these processes may allow us to manipulate these species so that they become more amenable to T-DNA-mediated genetic transformation.

9630779 Gelvin农杆菌Tumefaciens引起了许多植物的疾病冠军。冠状胆囊肿瘤发生的分子机制涉及细菌细胞与植物细胞壁的结合，以及对Ti（肿瘤诱导）质粒的区域的加工以及该T-（转移）DNA与植物细胞的转移。 Ti质粒已被修改为用作植物基因工程的载体。由于研究了二十年，我们对细菌基因和蛋白质对T-DNA转移和肿瘤发生的贡献了解很多。但是，我们对植物基因和蛋白质在这些过程中的作用一无所知。为了确定宿主编码的因素，农杆菌结合，T-DNA转移和整合以及植物激素信号转导所必需的因素，Gelvin博士最近筛选了不同的拟南芥生态型和T-DNA插入突变体的不同拟南芥插入突变体，以使其对冠状肿瘤的敏感性以及对其他T-DNA-and the其他功能的能力均表达了其他功能。在该研究项目中，Gelvin博士提出的实验是针对拟南芥T-DNA插入突变体中封闭的冠状胆囊肿瘤发生的步骤，这些步骤已被鉴定出来。这些宿主基因的表征不仅会增加我们对冠胆肿瘤发生的理解，而且还将有助于我们对碱性植物细胞生物学的了解。最后，对这些过程中涉及的宿主基因的理解和分离可能使我们能够操纵这些物种，从而使它们变得更适合T-DNA介导的遗传转化。