RIMI: Molecular Systematics of the Rotifera

RIMI：轮虫的分子系统学

• 批准号: 9628568
• 负责人: Elizabeth Walsh
• 金额: $ 24.96万
• 依托单位: University of Texas at El Paso
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9628568&HistoricalAwards=false
• 关键词: RIMI; Molecular; Systematics; Rotifera

HRD 9628730 New Mexico State University -- Bernard J. McNamara The proposed research will (1) investigate the long term behavior and energetics of the low mass X- ray binary (LMXB) Sco X-1 (2) recruit highly qualified minority undergraduates into the space sciences (3) involve minority undergraduate science and engineering students in two NASA programs and (4) recruit two undergraduate minority students into the astronomy program at NMSU . The project will use X-ray data obtained by the Compton Gamma- Ray Observatory and the X-ray Timing Explorer as well as optical data obtained at Cerro Tololo InterAmerican Observatory and New Mexico State University. The primary research goal is to obtain a multi-year record of the high energy flux and x-ray color of the LMXB Sco X-1 to search for expected long term emission variations from this system. The high energy data sets will also be combined with optical data to better understand this system s energetics. Abstract HRD -9628568 University of Texas at El Paso ---E.Walsh This RIMI project will attempt to provide a cladistic phylogeny of the major planktonic families using a molecular approach. The goals of the proposed research are to (1) isolate, amplify and sequence nuclear ribosomal DNA from representatives of 13 families of the monogonont rotifers, (2) determine the sister group of the monogononts by obtaining comparable data from four bdelloids and a seisonid, (3) construct cladograms and develop phylogenetic hypotheses using molecular characters (4) compare these results with tree topologies derived from morphological characters and (5) evaluate the placement of the Rotifera within the ex isting metazoan phylogenies based on 18S rDNA sequences. The phylogenetic hypotheses generated by the proposed research can be used to further resolve relationships among rortifers. They will constitute a basis from which to incorporate a historical component into understanding questions of broader evolutionary significance. In addition, the relationships among rotifers and other pseudo coelomate taxa will be explored. ABSTRACT HRD- 9628726 City College of C.U.N.Y. ----Moore The long term objective of this laboratory research project is to determine mechanisms cells use to maintain normal DNA function. Mutants will be studied to help determine these requirements. The RIMI project will focus on the characterizations mutants isolated in the laboratory on the basis of their hypersensitivities to killing by ionizing radiation, H2O2 , and a radiomimetic family of chemical congeners. This approach will acquire knowledge from interrelated studies at the cellular and molecular levels, using genetic characterizations, molecular biology and biochemistry, DNA sequence analyses, methodologies for studying DNA damage and repair / transmission and scanning electron microscopy. Cloning and characterizing the blm mutants will enable their relationship to already characterized genes to be determined and seems likely to add new knowledge of general importance to fields of DNA repair , metabolism and genetics. A variety of research training experiences will be provided for students at all levels of education including postdoctoral, graduate, undergraduate, and high school. ABSTRACT HRD---9628640 Meharry Medical College---S.Williams This RIMI project will support the establishment of a core facility in computational molecular biology consisting of state of the art equipment and software ( GCG Package and Molecular Simulations Protein Modeling Programs ), and provide training in their use. This facility will be available to all Meharry researchers. Specific research projects critically dependent on nucleic acid and protein analyses to be directly supported by this initiative are (1) identification of motifs that contribute to structure and function of hetero chromatin; (2) analysis of primary structure and folding patterns of RNA and its effects on viral recombination and replication; and (3) analysis of structural effects of site- directed mutations on flavin-containing monooxygenase. ABSTRACT HRD---9628388 Howard University ---M. McKenna Support is sought for the acquisition and installation of controlled environment growth chambers in the biology department greenhouse. The addition of these chambers will significantly increase research capabilities in plant science, aid in recruitment of talented undergraduates , graduate students and new faculty, and provide a facility that can support precollege outreach programs designed to increase the entry of minority students in science careers. The installation of controlled environment chambers will extend and enhance ongoing research in the areas of plant anatomy and development, phytoremediation, plant ecophysiology, plant ecology , plant systematics, mycology, plant molecular biology and genetics . The facility will allow the institution to maintain experimental plant populations over long periods, maintain clones or plant families for genetic experiments , grow source material for cellular and molecular experiments including tissue culture, DNA analysis, and phytoremediation, maintain plants for systematic study , and design experiments under controlled environmental conditions to study cellular, physiological or ecological processes. ABSTRACT HRD ----9628514 University of Texas at San Antonio----D.Jaffe The primary goal of this RIMI project is to expand the research capabilities of researchers at UTSA with a state of the art computer facility that will satisfy specific needs in molecular sequence analysis and three dimensional image processing. Studies that utilize molecular biological methods regularly produce data that need to be analyzed by comparison against large databases of existing sequences for similarities. pattern searching algorithms are used to unveil nonrandom sequence features that may be of biological relevance. Two computer workstations will be used to carry out molecular sequence analysis using the Intelligenetics Suite, a comprehensive molecular biology software package providing access to sequence databases worldwide and a broad spectrum of sequence analysis tools. The second half of the Computational Biology Facility will focus on cellular anatomy and physiology. Characterization of cellular morphology is critical, for understanding physiological function. Confocal microscopy permits the examination of cellular morphology in three dimensions. In addition

HRD 9628730 新墨西哥州立大学 -- Bernard J. McNamara 拟议的研究将 (1) 调查低质量 X 射线双星 (LMXB) Sco X-1 的长期行为和能量学 (2) 招募高素质的少数族裔本科生空间科学 (3) 让少数族裔本科生参与 NASA 的两个项目，(4) 招收两名少数族裔本科生加入新墨西哥州立大学的天文学项目。 该项目将使用康普顿伽马射线天文台和X射线计时探测器获得的X射线数据以及托洛洛山美洲天文台和新墨西哥州立大学获得的光学数据。主要研究目标是获得 LMXB Sco X-1 的高能通量和 X 射线颜色的多年记录，以寻找该系统预期的长期发射变化。高能数据集还将与光学数据相结合，以更好地了解该系统的能量学。 摘要 HRD -9628568 德克萨斯大学埃尔帕索分校 ---E.Walsh 该 RIMI 项目将尝试使用分子方法提供主要浮游生物科的分支系统发育。拟议研究的目标是 (1) 对 13 个单角轮虫科代表的核核糖体 DNA 进行分离、扩增和测序，(2) 通过从四种蛭形动物和一种轮虫中获得可比较的数据来确定单角轮虫的姐妹群， (3) 使用分子特征构建分支图并提出系统发育假设 (4) 将这些结果与源自形态特征的树拓扑进行比较，以及 (5) 评估基于 18S rDNA 序列的现有后生动物系统发育中的轮虫。本研究产生的系统发育假设可用于进一步解决轮嘴虫之间的关系。它们将构成一个基础，在此基础上将历史成分纳入理解具有更广泛进化意义的问题。 此外，还将探讨轮虫和其他拟腔皮动物类群之间的关系。 摘要 HRD- 9628726 纽约市立大学城市学院----Moore 该实验室研究项目的长期目标是确定细胞用于维持正常 DNA 功能的机制。将研究突变体以帮助确定这些要求。 RIMI 项目将重点研究实验室分离的突变体的特征，这些突变体对电离辐射、H2O2 和化学同系物的拟辐射家族具有超敏性。这种方法将从细胞和分子水平的相关研究中获取知识，利用遗传特征、分子生物学和生物化学、DNA序列分析、研究DNA损伤和修复/传输的方法以及扫描电子显微镜。克隆和表征 blm 突变体将能够确定它们与已表征基因的关系，并且似乎可能为 DNA 修复、代谢和遗传学领域增加具有普遍重要性的新知识。将为博士后、研究生、本科生、高中等各个教育层次的学生提供各种研究培训经历。 摘要 HRD---9628640 梅哈里医学院---S.Williams 该 RIMI 项目将支持建立计算分子生物学核心设施，包括最先进的设备和软件（GCG 软件包和分子模拟蛋白质建模程序），并提供使用培训。该设施将向所有梅哈里研究人员开放。该计划直接支持的主要依赖于核酸和蛋白质分析的具体研究项目包括：（1）鉴定有助于异染色质结构和功能的基序； （2）RNA一级结构和折叠模式分析及其对病毒重组和复制的影响； (3)定点突变对含黄素单加氧酶的结构影响的分析。 摘要HRD---9628388 霍华德大学---M。寻求麦肯纳支持在生物系温室中购买和安装受控环境生长室。这些室的增加将显着提高植物科学的研究能力，帮助招募有才华的本科生、研究生和新教师，并提供一个可以支持旨在增加少数族裔学生进入科学职业的大学预科推广项目的设施。受控环境室的安装将扩展和加强植物解剖学和发育、植物修复、植物生态生理学、植物生态学、植物系统学、真菌学、植物分子生物学和遗传学等领域正在进行的研究。该设施将使该机构能够长期维持实验植物种群，维持用于基因实验的克隆或植物家族，为细胞和分子实验（包括组织培养、DNA 分析和植物修复）种植源材料，维持植物以进行系统研究和设计在受控环境条件下进行实验，以研究细胞、生理或生态过程。 摘要 HRD ----9628514 德克萨斯大学圣安东尼奥分校----D.Jaffe 该 RIMI 项目的主要目标是通过满足特定需求的最先进的计算机设施来扩展 UTSA 研究人员的研究能力分子序列分析和三维图像处理。利用分子生物学方法的研究经常产生数据，需要通过与现有序列的大型数据库进行比较来分析相似性。模式搜索算法用于揭示可能具有生物学相关性的非随机序列特征。两台计算机工作站将用于使用 Intelligenics Suite 进行分子序列分析，这是一个全面的分子生物学软件包，可提供对全球序列数据库的访问和广泛的序列分析工具。计算生物学设施的后半部分将重点关注细胞解剖学和生理学。细胞形态的表征对于理解生理功能至关重要。 共焦显微镜可以在三个维度上检查细胞形态。此外