Role of the CRINKLY4 Receptor Kinase in Plant Epidermal Differentiation

CRINKLY4 受体激酶在植物表皮分化中的作用

基本信息

  • 批准号:
    9604426
  • 负责人:
  • 金额:
    $ 49.49万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    1997
  • 资助国家:
    美国
  • 起止时间:
    1997-04-01 至 2002-03-31
  • 项目状态:
    已结题

项目摘要

Becraft 96-04426 Plant leaves are covered by a highly specialized epidermal layer which functions in development to delimit organs and prevent organ fusions. The endosperm of grass seeds also contains an epidermis-like cell layer called the aleurone which secretes hydrolytic enzymes required for remobilization of storage compounds during germination. The crinkly4 (cr4) gene of maize is involved in the differentiation of leaf epidermis and aleurone. cr4 encodes a receptor kinase with extracellular similarity to the ligand binding domain of tumor necrosis factor receptors (TNFRs) from mammals. Tumor necrosis factors (TNFs) are small protein signal molecules that are involved in developmental processes in the immune system and other tissues. The similarity between CR4 and TNFR suggests that the ligand for CR4 might also be a protein of the TNF class. Genomic southern hybridization suggests that cr4 is a member of a gene family. The proposed research has three specific aims: 1. Complete a molecular genetic analysis of the maize cr4 gene. This includes generating an allelic series of cr4 mutants and analyzing the expression pattern of the cr4 gene. The expression pattern will be studied using immunohistochemistry and genetic mosaic analyses. 2. Isolate a ligand for the extracellular domain of CR4. Three different approaches are proposed: A. molecular identification of a cloned gene that encodes a protein able to bind the extracellular domain of CR4 (i.e. interaction cloning), B. genetic identification of an interacting protein by analysis of mutants with similar phenotypes to cr4, or by identification of second site mutations that enhance or suppress the cr4 mutant phenotype, and C. a transgenic approach to introduce specifically engineered mutations that can be used in genetic suppressor screens. 3. Characterize the cr4 gene family in arabidopsis to determine the extent of the family, whether all members encode receptor kineses and the expression profiles for each member.
Becraft 96-04426 植物叶子被高度特化的表皮层覆盖,该表皮层在发育过程中发挥着界定器官和防止器官融合的作用。草籽的胚乳还含有称为糊粉层的表皮样细胞层,其分泌在发芽过程中重新动员储存化合物所需的水解酶。玉米的 crinkly4 (cr4) 基因参与叶表皮和糊粉层的分化。 cr4 编码的受体激酶与哺乳动物肿瘤坏死因子受体 (TNFR) 的配体结合域具有细胞外相似性。肿瘤坏死因子 (TNF) 是参与免疫系统和其他组织发育过程的小蛋白质信号分子。 CR4 和 TNFR 之间的相似性表明 CR4 的配体也可能是 TNF 类蛋白质。基因组 Southern 杂交表明 cr4 是基因家族的成员。拟议的研究有三个具体目标: 1. 完成玉米 cr4 基因的分子遗传分析。这包括生成 cr4 突变体的等位基因系列并分析 cr4 基因的表达模式。将使用免疫组织化学和遗传镶嵌分析来研究表达模式。 2. 分离 CR4 胞外结构域的配体。提出了三种不同的方法:A. 编码能够结合 CR4 胞外域的蛋白质的克隆基因的分子鉴定(即相互作用克隆),B. 通过分析与 cr4 具有相似表型的突变体来遗传鉴定相互作用蛋白质,或通过鉴定增强或抑制cr4突变体表型的第二位点突变,以及C.引入可用于基因抑制筛选的专门工程突变的转基因方法。 3. 表征拟南芥中的 cr4 基因家族,以确定该家族的范围、是否所有成员都编码受体动力学以及每个成员的表达谱。

项目成果

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Philip Becraft其他文献

Philip Becraft的其他文献

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{{ truncateString('Philip Becraft', 18)}}的其他基金

CONFERENCE: 55th Annual Maize Genetics Conference to be held March 14-17, 2013; St. Charles, Illinois
会议:第 55 届年度玉米遗传学会议将于 2013 年 3 月 14-17 日举行;
  • 批准号:
    1302963
  • 财政年份:
    2013
  • 资助金额:
    $ 49.49万
  • 项目类别:
    Standard Grant
Transcriptional Regulation of Maize Endosperm Development by IDD9 and IDDveg9 Duplicate Genes
IDD9 和 IDDveg9 重复基因对玉米胚乳发育的转录调控
  • 批准号:
    1121738
  • 财政年份:
    2011
  • 资助金额:
    $ 49.49万
  • 项目类别:
    Continuing Grant
Conference: Plant Receptor Signaling at Iowa State University.
会议:爱荷华州立大学植物受体信号转导。
  • 批准号:
    0614938
  • 财政年份:
    2006
  • 资助金额:
    $ 49.49万
  • 项目类别:
    Standard Grant
Analysis of the Plant CR4 Receptor Kinase and Gene Family
植物 CR4 受体激酶和基因家族分析
  • 批准号:
    9634145
  • 财政年份:
    1996
  • 资助金额:
    $ 49.49万
  • 项目类别:
    Standard Grant
Postdoctoral Research Fellowship in Plant Biology
植物生物学博士后研究奖学金
  • 批准号:
    9203709
  • 财政年份:
    1992
  • 资助金额:
    $ 49.49万
  • 项目类别:
    Fellowship Award
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