A Rotating Anode X-ray Generator for Biological Macromolecular Crystallography

用于生物大分子晶体学的旋转阳极X射线发生器

• 批准号: 9604319
• 负责人: Leemor Joshua-Tor
• 金额: $ 8.41万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 1998-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604319&HistoricalAwards=false
• 关键词: Rotating; Anode; ray; Generator; Biological

Funds are requested to purchase a rotating anode X-ray generator in order to measure diffraction data from biological macromolecular crystals. This instrument will provide a source of X-rays urgently needed for our existing imaging plate detector system and will directly support the research of 3 major investigators as well as instruction in protein crystallography. Projects that will utilize this X-ray source include DNA methylation; the bleomycin hydrolases and their complexes; transcription complexes; and RNA splicing factors, for all of which crystals are currently available. The crystal structures elucidated in the course of these projects will provide structural frameworks, along with the biochemical and genetic studies, for interpreting the functional and molecular properties of this diverse range of biochemically and biomedically significant macromolecules. In addition, the equipment is necessary for teaching an advanced course in macromolecular crystallography offered in the fall of every year at Cold Spring Harbor Laboratory as well as training our own graduate students and postdoctoral fellows. The advanced course in macromolecular crystallography has a wide impact on the field of structural biology and thus in addition to its importance for the specific crystallographic studies described here, the value of a properly operating X-ray facility to the field of macromolecular crystallography in general would be substantial.

要求资金购买旋转的阳极X射线发生器，以测量从生物大分子晶体的衍射数据。该仪器将为我们现有的成像板探测器系统急需提供X射线源，并将直接支持3位主要研究人员的研究以及蛋白质晶体学的指导。利用此X射线来源的项目包括DNA甲基化；博来霉素水解酶及其复合物；转录复合物；和RNA剪接因子，目前可用的所有晶体。 在这些项目过程中阐明的晶体结构将提供结构框架，以及生化和遗传研究，用于解释这种多样的生化和生物医学重要的大分子的功能和分子特性。此外，该设备对于每年在冷泉港实验室秋天提供的大分子晶体学的高级课程以及培训我们自己的研究生和博士后研究员所需的设备是必不可少的。 大分子晶体学的高级课程对结构生物学领域具有广泛的影响，因此除了其对此处描述的特定晶体学研究的重要性外，正确运行的X射线设备对大分子晶体学领域的价值一般还很重要。