Ecological and Evolutionary Genetics of the S-Locus in the Solanaceae

茄科 S 基因座的生态和进化遗传学

• 批准号: 9527834
• 负责人: Joshua Kohn
• 金额: $ 18.3万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-15 至 2000-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9527834&HistoricalAwards=false
• 关键词: Ecological; Evolutionary; Genetics; Locus; Solanaceae

9527834 KOHN The use of a novel molecular approach will provide detailed information on the self-incompatibility (S) locus, which controls the rejection of self pollen and acceptance of cross pollen in members of the tomato family (Solanaceae). This genetic locus controls a cellular system of self-recognition and rejection that is only partially understood at present. DNA sequence information at this locus has so far been limited to rather few, highly divergent, alleles from widely separated species. The S-locus has been little studied at the population level because current technology for determining the genotype of individual plants requires the use of cumbersome crossing methods. Our molecular approach decreases the time needed to sample S-allele diversity within and between populations and provides detailed molecular information from closely related alleles for studying the relationship between DNA sequence change and rejection specificity. Information on allele number will be used to relate species ecological characteristics to effective population size, a quantity of concern to conservation biologists. Genealogical structure of S-alleles will be used to measure the frequency of historical bottlenecks and test the frequency of one possible mechanism for species formation. Test crosses among plants carrying S-alleles with very similar molecular sequences will help resolve the relationship between sequence variation and the ability to recognize self from non-self pollen. Understanding how sequences control rejection specificity will provide information useful in plant breeding where self-incompatibility limits the breeder's ability to create homozygous inbred lines.

9527834 KOHN的使用新型分子方法将提供有关自我兼容性基因座的详细信息，该基因座控制了自我花粉的排斥和接受番茄家族成员（Solanaceae）的跨花粉的接受。这个遗传基因座控制着一种自我识别和排斥的细胞系统，目前仅部分理解。 到目前为止，该基因座的DNA序列信息仅限于很少有来自广泛分离物种的高等等位基因。 S-Locus在人群水平上很少研究，因为当前用于确定单个植物基因型的技术需要使用繁琐的穿越方法。我们的分子方法减少了种群内和种群之间的S-贵族多样性所需的时间，并从密切相关的等位基因中提供了详细的分子信息，以研究DNA序列变化与拒绝特异性之间的关系。 有关等位基因数量的信息将用于将物种生态特征与有效的人口规模联系起来，这是保护生物学家的一定数量。 S-Cleares的家谱结构将用于测量历史瓶颈的频率，并测试一种物种形成的一种可能机制的频率。携带具有非常相似分子序列的S- leacer的植物之间的测试杂交将有助于解决序列变化与识别非自身花粉中自我的能力之间的关系。了解序列如何控制拒绝特异性将为植物繁殖提供有用的信息，在这种信息中，自我兼容性限制了育种者创建纯合子繁殖线的能力。