Electroporation and Electroporation Assisted Disinfection for Inactivation of Giardia and Cryptosporidium in Drinking Water

电穿孔和电穿孔辅助消毒灭活饮用水中贾第鞭毛虫和隐孢子虫

• 批准号: 9504835
• 负责人: Charles Haas
• 金额: $ 17.35万
• 依托单位: Drexel University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9504835&HistoricalAwards=false
• 关键词: Electroporation; Assisted; Disinfection; Inactivation; Giardia

9504835 Haas This is an award to support research the objective of which is to determine the efficacy of and optimal conditions for the use of a process known as electroporation as a direct inactivating agent or as a promoter of inactivation for microbial contaminants of water. The electroporation process as applied to inactivation of microorganisms suspended in a fluid involves subjecting the suspension to brief high-density voltage pulses produced by the decaying discharges from a resistance-capacitance electrical circuit. The investigator plans on conducting this research on a laboratory scale using cysts of Giardia muris and oocysts of Cryptosporidium parvum. The investigator plans on studying the effects of electroporation alone and as a potential promoter of inactivation in the presence of other disinfectants such as ozone, monochloramine and free chlorine. Results of this research are expected to identify conditions under which electroporation can be used alone as a process for disinfection of water that has been contaminated by organisms exemplified by those to be studied in this project. Positive results in that respect would provide the basis for the potential use of this process in environmental engineering practice with special emphasis on disinfection of drinking water. In addition, results are expected to provide insights into the structure and physiological properties of cysts and oocysts of potential use in basic microbiological or medical research.

9504835 Haas 该奖项旨在支持研究，其目的是确定使用电穿孔工艺作为直接灭活剂或作为水微生物污染物灭活促进剂的功效和最佳条件。 用于灭活悬浮在流体中的微生物的电穿孔过程涉及使悬浮液经受由电阻电容电路的衰减放电产生的短暂高密度电压脉冲。 研究人员计划利用鼠贾第鞭毛虫的包囊和小隐孢子虫的卵囊在实验室规模上进行这项研究。 研究人员计划研究单独使用电穿孔的效果以及在存在其他消毒剂（例如臭氧、一氯胺和游离氯）的情况下作为灭活的潜在促进剂的效果。 这项研究的结果预计将确定在哪些条件下可以单独使用电穿孔作为对已被本项目中要研究的生物体污染的水进行消毒的方法。 这方面的积极成果将为该工艺在环境工程实践中的潜在应用奠定基础，特别是饮用水消毒。 此外，预计结果将有助于深入了解包囊和卵囊的结构和生理特性，这些包囊和卵囊在基础微生物学或医学研究中具有潜在用途。