Heterochromatic Loci and Position Effects in Drosophila

果蝇的异染色质位点和位置效应

• 批准号: 9406691
• 负责人: Barbara Wakimoto
• 金额: $ 9.84万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9406691&HistoricalAwards=false
• 关键词: Heterochromatic; Loci; Position; Effects; Drosophila

9406691 Wakimoto The chromosomes of higher eukaryotes are comprised of two cytologically distinct domains known as euchromatin and heterochromatin. These regions differ in the extent of condensation, with heterochromatin remaining tightly condensed throughout the cell cycle.The structure and function of heterochromatin will be examined through the study of a set of genes that reside within the heterochromatin on the left arm of Chromosome 2 (2LH) of Drosophila melanogaster. Specific repetitive DNAs isolated from 2LH will be tested for the ability to confer the properties of heterochromatin and will be assayed for effects on chromosome morphology, heterochromatic and euchromatic gene expression and gene dosage using germline transformation and chromosome rearrangements. %%% The long range objective of the proposed project is to define the molecular determinants of higher order chromosome structure and the functional significance of heterochromatin. The structure and function of heterochromatin will be examined by inserting heterochromatic repetitive DNA sequences into the genome of the fruit fly and assaying for effects on morphology, gene expression and gene dosage. ***

9406691 Wakimoto 高等真核生物的染色体由两个细胞学上不同的结构域组成，称为常染色质和异染色质。这些区域的浓缩程度不同，异染色质在整个细胞周期中保持紧密浓缩。通过研究位于 2 号染色体左臂异染色质 (2LH) 内的一组基因，将检查异染色质的结构和功能。 ）的果蝇。从 2LH 中分离的特定重复 DNA 将被测试赋予异染色质特性的能力，并将使用种系转化和染色体重排来分析对染色体形态、异染色质和常染色质基因表达以及基因剂量的影响。 %%% 该项目的长期目标是定义高级染色体结构的分子决定因素和异染色质的功能意义。通过将异染色质重复 DNA 序列插入果蝇基因组并分析对形态、基因表达和基因剂量的影响，可以检查异染色质的结构和功能。 ***