Regulation of Glyoxysome Formation During Higher Plant Development

高等植物发育过程中乙醛酸酶体形成的调控

基本信息

批准号：
9317526
负责人：
John Harada
金额：
$ 37.9万
依托单位：
University of California-Davis
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1994
资助国家：
美国
起止时间：
1994-04-15 至 1998-03-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317526&HistoricalAwards=false
关键词：
Regulation Glyoxysome Formation During Higher

项目摘要

9317526 Harada The long range scientific role of this project is to obtain a mechanistic understanding of the processes that specify the function and structure of glyoxysomes, subcellular organelles that compartmentalize critical metabolic reactions in a cell. In this proposal, two key questions about glyoxysome formation are considered. First, how is the induction of glyoxysomal function regulated during plant development? This question will be addressed by dissecting the processes regulating genes encoding two glyoxysome-specific enzymes, isocitrate lyase and malate synthase, that are required for glyoxysomal function. Previous studies from this lab established that transcriptional processes play the primary role in regulating accumulation of these enzymes in glyoxysomes. Therefore, a mechanistic understanding of the processes regulating expression of these two genes will provide insight into the physiological signal(s) and pathway(s) that control glyoxysomal function during plant development. The second key question will focus on glyoxysome assembly. What are the genes that are required to establish the structure of the organelle and to import proteins into peroxisomes, and how are these genes regulated during development? Genes involved in glyoxysome assembly will be identified by selecting plant cDNA clones that complement mutants of Saccharomyces cerevisiae. In the long term, these approaches will provide a global overview of the processes involved in glyoxysome formation. The proposed studies will provide significant basic information about the processes that establish an organelle's structure and function. Furthermore, infomation obtained from these studies may be directly applicable to the design of strategies to improve crop plants using genetic engineering protocals. ***

9317526原田该项目的远距离科学作用是对指定糖化体功能和结构的过程的机械理解，这些过程和结构是细胞内细胞内细胞器，这些细胞器会使细胞中关键的代谢反应分裂。在此提案中，考虑了有关糖明组形成的两个关键问题。首先，在植物发育过程中如何调节乙氧化氧学功能的诱导？该问题将通过解剖调节编码两种乙二醇裂解酶和苹果酸合酶的调节基因的过程，这些酶是乙酰氧化液所需的。该实验室的先前研究表明，转录过程在调节这些酶在糖体中的积累中起主要作用。因此，对调节这两个基因表达的过程的机理理解将提供对控制植物发育过程中控制乙醛酸函数的生理信号和途径的见解。第二个关键问题将集中在乙二醇组合体上。建立细胞器结构并将蛋白质导入过氧化物酶体所需的基因是什么？在发育过程中如何调节这些基因？通过选择补充酿酒酵母突变体的植物cDNA克隆，将鉴定参与糖明组装的基因。从长远来看，这些方法将提供全球概述，概述乙二醇组形成所涉及的过程。拟议的研究将提供有关建立细胞器结构和功能的过程的重要基本信息。此外，从这些研究中获得的信息可能直接适用于使用基因工程质子来改善作物植物的策略的设计。 ***