Cyanophages of Marine Synechococcus: A Model System to Evaluate the Impact of Viruses on the Population Structure and Dynamics of Planktonic Bacteria

海洋聚球藻的噬藻体：评估病毒对浮游细菌种群结构和动态影响的模型系统

• 批准号: 9315895
• 负责人: John Waterbury
• 金额: $ 35.86万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-15 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9315895&HistoricalAwards=false
• 关键词: Cyanophages; Marine; Synechococcus; Model; System

Waterbury 9315895 Previous studies by this investigator have confirmed that the presence of a diverse group of marine cyanobacterial phages that are capable of infecting Synechococcus and that they can occur at extremely high titers of 104 phage particles per milliliter. In addition, Waterbury has shown that a natural bacterial population and its phages follow the patterns predicted from modeling and chemostat studies of E. coli and its virulent phages. Thus, Waterbury hypothesizes that due to the acquisition of resistance, virulent Synechococcus phages play an important role in determining the clonal composition of natural Synechococcus populations, but that they have little effect on the overall population densities or mortality. To test this hypothesis, a laboratory project is designed to develop ribonucleic acid and antibody probes to follow the species dynamics of Synechococcus and its phages, respectively. This host-parasite system will prove to be an excellent model to examine the role of bacteriophages in the oceans. ***

Waterbury 9315895 该研究人员之前的研究已证实存在多种能够感染聚球藻的海洋蓝藻噬菌体，并且它们的滴度极高，可达每毫升 104 个噬菌体颗粒。 此外，沃特伯里还表明，天然细菌群体及其噬菌体遵循大肠杆菌及其剧毒噬菌体的建模和恒化器研究预测的模式。 因此，沃特伯里假设，由于获得了抗性，有毒的聚球藻噬菌体在确定天然聚球藻种群的克隆组成方面发挥着重要作用，但它们对总体种群密度或死亡率影响不大。 为了检验这一假设，一个实验室项目旨在开发核糖核酸和抗体探针，以分别跟踪聚球藻及其噬菌体的物种动态。这种宿主-寄生虫系统将被证明是研究噬菌体在海洋中的作用的绝佳模型。 ***