The Screening of Mutagenized abi3 Seeds of Arabidopsis thaliana for New Giberellin Sensitivity Mutants, and their Characterization

诱变拟南芥 abi3 种子新赤霉素敏感性突变体的筛选及其表征

• 批准号: 9307067
• 负责人: Valerie Sponsel
• 金额: $ 3.07万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9307067&HistoricalAwards=false
• 关键词: Screening; Mutagenized; abi3; Seeds; Arabidopsis

9307067 Sponsel The proposed research involves a new screening procedure for mutagenised Arabidopsis thaliana seeds to select plant stature mutants which differ in their ability synthesize or respond to the bibberellin (GA) group of plant hormones. Since GAs are normally required to counteract abscisic acid (ABA)-induced seed dormancy in Arabidopsis the original mutagenesis will be conducted using seeds which are homozygous recessive at the AB13 locus. The ab13 allele causes insensitivity to ABA and hence prevents seed dormancy. It is expected that mutations which perturb the biosynthesis or response to GA, and which would normally prevent seed germination, will not impair germination in ab13 seeds. Therefore this screening protocol should allow the isolation of new GA mutants, especially new GA-response mutants. During the twelve months of this award it is proposed to begin the genetic analysis of these mutants in association with Dr. M. Estelle. The biochemical analysis will also be initiated. It will involved the identification and qualification of GAs in the new mutants using combined gas chromatography-mass spectrometry. The characterization of new GA-insensitive mutants in which the reception of the GA signal, or the tranduction of that signal into a growth response is impaired will assist in the identification of genes which are involved in these events.

9307067 Sponsel 拟议的研究涉及对诱变拟南芥种子进行新的筛选程序，以选择合成或响应比柏林 (GA) 类植物激素的能力不同的植物身材突变体。 由于拟南芥中通常需要 GA 来抵消脱落酸 (ABA) 诱导的种子休眠，因此最初的诱变将使用 AB13 基因座纯合隐性的种子进行。 ab13 等位基因导致对 ABA 不敏感，从而阻止种子休眠。 预计扰乱生物合成或对 GA 的反应以及通常会阻止种子发芽的突变不会损害 ab13 种子的发芽。 因此，该筛选方案应允许分离新的 GA 突变体，尤其是新的 GA 响应突变体。 在该奖项的十二个月内，建议与 M. Estelle 博士合作开始对这些突变体进行遗传分析。 生化分析也将启动。 它将涉及使用气相色谱-质谱联用技术对新突变体中的 GA 进行鉴定和鉴定。 新的 GA 不敏感突变体（其中 GA 信号的接收或将该信号转导为生长反应受到损害）的表征将有助于鉴定参与这些事件的基因。