RUI: Acquisition of equipment for tracking and purifying radioactive proteins

RUI：采购用于跟踪和纯化放射性蛋白质的设备

• 批准号: 9220666
• 负责人: Maria Linder
• 金额: $ 3.01万
• 依托单位: CSU Fullerton Auxiliary Services Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9220666&HistoricalAwards=false
• 关键词: RUI; Acquisition; equipment; tracking; purifying

The objective of this proposal is to acquire a high quality, automatic FPLC system and a new gamma counter, to purify and track proteins/peptides (particularly radioactive proteins/peptides) and their metals or prosthetic groups. The FPLC system is needed because the laboratories of the primary investigators currently have only very limited access to such systems, although in each, purification of proteins/peptides (for determination of their structure, function and regulation) is a major activity. A new gamma counter is needed because the existing one is twelve years old and threatening to become unreliable. If it should fail, this would greatly limit the research of the primary investigators, who are utterly dependent upon it for tracing copper and iron in biological systems; tracking copper and iron binding proteins and their receptors during their purification and/or in studies of their binding capacities, kinetics and functions; identifying the binding sites of substrates and cofactors of specific enzymes via affinity labeling. The isotopes used (67Cu,64Cu,59Fe, 125I) are all gamma emitters, and double labeling is often required.

该提案的目标是获得高质量的自动 FPLC 系统和新的伽马计数器，以纯化和跟踪蛋白质/肽（特别是放射性蛋白质/肽）及其金属或辅基。 FPLC 系统是必要的，因为主要研究人员的实验室目前对此类系统的访问非常有限，尽管在每个系统中，蛋白质/肽的纯化（用于确定其结构、功能和调节）是主要活动。 需要一台新的伽玛计数器，因为现有的伽玛计数器已经使用了 12 年并且有变得不可靠的危险。 如果它失败了，这将极大地限制主要研究人员的研究，他们完全依赖它来追踪生物系统中的铜和铁；在其纯化过程中和/或在其结合能力、动力学和功能的研究过程中追踪铜和铁结合蛋白及其受体；通过亲和标记识别特定酶的底物和辅因子的结合位点。 使用的同位素（67Cu、64Cu、59Fe、125I）都是伽马发射体，通常需要双重标记。