Enzyme and Antibody Catalysts for Polyene Cyclization Reactions

用于多烯环化反应的酶和抗体催化剂

• 批准号: 9018241
• 负责人: John Griffin
• 金额: $ 3.67万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018241&HistoricalAwards=false
• 关键词: Enzyme; Antibody; Catalysts; Polyene; Cyclization

This award is made as the starter grant increment of Dr. John Griffin's Postdoctoral Fellowship in Chemistry Award in support of his research at Stanford University. The thrust of Dr. Griffin's research is the isolation, purification and characterization of enzyme and antibody catalysyts for polyene cyclization reactions. Enzymatic activity of purified cyclase will be quantified and the amino acid and gene sequence will be determined. The cyclizing acive site will be studied through affinity labelling and directed mutagenesis experiments. This should permit the design and preparation of engineered enzymes having novel substrate and/or product specificities. Heterobicyclic immunogens will be synthesized which will elicit antibodies capable of initiating, directing and terminating the cyclization of diene epoxides. Once antibody catalysts have been identified, their mechanism of action will be studied. This should lead to a better understanding of sterol biosynthesis and macromolecular catalysis and may provide useful catalysts for the synthesis of sterols.

该奖项是约翰·格里芬博士（John Griffin）博士的化学博士学位奖学金奖的首发赠款，以支持他在斯坦福大学的研究。格里芬博士的研究的推力是酶和抗体催化反应的分离，纯化和表征。 将定量纯化环化酶的酶活性，并确定氨基酸和基因序列。将通过亲和力标记和定向诱变实验来研究环化的活位点。 这应该允许设计和制备具有新型底物和/或产品特异性的工程酶。将合成杂环免疫原子，这将引起能够引发，指导和终止二烯环氧化物环化的抗体。一旦鉴定出抗体催化剂，将研究其作用机理。 这应该可以更好地了解固醇生物合成和大分子催化，并可能为合成固醇的合成提供有用的催化剂。