Acquisition of a Spectrofluorometer

购买荧光分光光度计

基本信息

批准号：
9016177
负责人：
Peter Holloway
金额：
$ 3.8万
依托单位：
University of Virginia Main Campus
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1991
资助国家：
美国
起止时间：
1991-01-01 至 1992-12-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9016177&HistoricalAwards=false
关键词：
Acquisition Spectrofluorometer

Acquisition Spectrofluorometer

项目摘要

The group of investigators listed in this proposal have developed, over the years, many techniques for examining various aspects of membrane structure and function. Steady state fluorescence measurements are a fundamental measurement which is ideally suited to membranous systems and this application will describe several diverse techniques which have been, and will be, used to investigate a series of membrane problems from the most fundamental lipid-lipid interaction to the most complex of cell signalling processes. These projects include: the formation of compositional lipid domains in multilipid systems, the conformation of membrane-bound proteins, the mechanism of protein-lipid interactions, the role of lipid physical properties in the functioning of an integral membrane protein and the mechanism of intracellular signalling. In many of the projects involving membrane proteins, advantage will be taken of site-specific mutagenesis to produce modified proteins with more favorable fluorescent properties.

多年来，该提案中列出的研究人员已经开发出许多用于检查膜结构和功能各个方面的技术。稳态荧光测量是一种基本测量值，非常适合膜系统，该应用将描述已经并且将被用来研究一系列膜问题，从最基本的脂质脂质相互作用到最复杂的细胞信号传导过程。这些项目包括：多脂质系统中成分脂质结构域的形成，膜结合蛋白的构象，蛋白质脂质相互作用的机制，脂质物理特性在整体膜蛋白的功能中的作用以及细胞内信号的机制。在许多涉及膜蛋白的项目中，将利用位点特异性诱变来产生具有更有利荧光性能的改良蛋白。