RUI: Nuclear Cytology and Centrin in the Red Algae

RUI：红藻中的核细胞学和中心蛋白

基本信息

批准号：
9008078
负责人：
Joseph Scott
金额：
$ 22.73万
依托单位：
College of William and Mary
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1990
资助国家：
美国
起止时间：
1990-09-01 至 1994-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9008078&HistoricalAwards=false
关键词：
RUI Nuclear Cytology Centrin Red

项目摘要

Drs. Joseph Scott and Sharon Broadwater of the College of William and Mary are studying the details of cell division in groups of red algae (Rhodophyta) using scanning and transmisson electron microscopy. The number, kinds, and orientations of subcellular structures combine to create patterns of cell division that appear to be characteristic for major groups of red algae at the ordinal or subclass taxonomic level. Research is continuing on cell division patterns in groups of Corallinales and Batrachospermales, which at present are poorly known. The investigators are also conducting a search in red algae for the recently described protein centrin, which is a contractile protein thought to be present in all eukaryotic cells and involved in chromosome movement. In addition, the enigmatic unicellular organism Glaucosphaera vacuolata is being studied through electron microscopy and molecular and immunocytochemical methods. One of the unique characters of Rhodophyta, alone among the world's algae, is the lack of a flagellar apparatus, yet Drs. Scott and Broadwater have preliminary data indicating that G. vacuolata possesses a non-emergent flagellum located within a red-algal cytoplasm. Molecular and immunocytochemical tests are being made with the help of collaborators, to confirm the presence of centrin and of a reduced flagellum. The results will provide new insights into phylogenetic relationships within the red algae and with other groups of primitive organisms.

博士。威廉玛丽学院的 Joseph Scott 和 Sharon Broadwater 正在使用扫描和透射电子显微镜研究红藻类（红藻门）细胞分裂的细节。亚细胞结构的数量、种类和方向结合起来形成了细胞分裂的模式，这些模式似乎是红藻主要类群在序数或亚类分类水平上的特征。关于珊瑚目和蝙蝠藻目细胞分裂模式的研究仍在继续，但目前人们对此知之甚少。研究人员还在红藻中寻找最近描述的中心蛋白，这是一种收缩蛋白，被认为存在于所有真核细胞中并参与染色体运动。此外，人们正在通过电子显微镜、分子和免疫细胞化学方法研究神秘的单细胞生物空泡青球菌（Glaucosphaera vacuolata）。红藻门是世界上唯一的藻类，它的独特特征之一是缺乏鞭毛装置，但 Drs. Scott 和 Broadwater 的初步数据表明，G. vacuolata 拥有位于红藻细胞质内的非涌现鞭毛。在合作者的帮助下正在进行分子和免疫细胞化学测试，以确认中心蛋白和减少的鞭毛的存在。这些结果将为红藻内部以及与其他原始生物群体的系统发育关系提供新的见解。