Hydrogen Tunneling in Enzyme Reactions

酶反应中的氢隧道

• 批准号: 8911632
• 负责人: Judith Klinman
• 金额: $ 22.18万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-15 至 1995-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8911632&HistoricalAwards=false
• 关键词: Hydrogen; Tunneling; Enzyme; Reactions

Recent studies from this laboratory have established that hydrogen tunneling occurs in the yeast alcohol dehydrogenase and bovine serum amine oxidase reactions. These initial findings suggest that room temperature tunneling may be fundamental feature of enzyme catatlyzed hydrogen transfer reactions. Investigation will be extended in order to: (i) Determine the generality of hydrogen tunneling in enzyme catalyzed H:, H. and H+ reactions, thereby establishing a data base for such behavior; (ii) Characterize the parameters which are likely to control the probability of tunneling, in particular the roles of reaction driving force, E.S binding interactions and protein motion; and (iii) Initiate computational work, with the goal of discriminating among possible models for nuclear tunneling at enzyme active sites. Ultimately, such studies are expected to provide insight into the shape of reaction coordinates at enzyme active sites. Over the past few years the role of quantum mechanical effects in enzyme reactions have gained importance. Although the area of electron tunneling has been the focus of extensive on going investigatrions the phenomenon of hydrogen tunneling in biology has received relatively little attention. It is a particular promising area to pursue to find answer too many features of enzyme catalysis.

该实验室最近的研究表明，酵母醇脱氢酶和牛血清胺氧化酶反应中会发生氢隧道效应。 这些初步发现表明室温隧道效应可能是酶催化氢转移反应的基本特征。 研究将扩大，以便： (i) 确定酶催化 H:、H. 和 H+ 反应中氢隧道效应的普遍性，从而建立此类行为的数据库； (ii) 表征可能控制隧道效应概率的参数，特别是反应驱动力、E.S结合相互作用和蛋白质运动的作用； (iii) 启动计算工作，目标是区分酶活性位点核隧道的可能模型。 最终，此类研究有望深入了解酶活性位点的反应坐标形状。 在过去的几年中，量子力学效应在酶反应中的作用变得越来越重要。 尽管电子隧道领域一直是广泛研究的焦点，但生物学中的氢隧道现象受到的关注相对较少。 这是一个特别有前途的领域，需要寻找酶催化的众多特征的答案。