Evaluation of Gene Probe Technology for the Detection of Human Immunodeficiency Virus in Hospital Wastewater Concen- trates

医院废水浓缩物中人类免疫缺陷病毒检测基因探针技术的评价

• 批准号: 8905210
• 负责人: Joan Rose
• 金额: $ 3万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8905210&HistoricalAwards=false
• 关键词: Evaluation; Gene; Probe; Technology; Detection

The objective of this project is to conduct exploratory research on filtration techniques for detection and recovery of the human immunodeficiency virus (HIV) and on the use of gene probe techniques for detection of the presence of this virus in water that has been in contact with solid and liquid wastes that are contaminated with this virus. In this preliminary research, recovery of the virus will be investigated by use of positively charged depth filters, a technique that has been shown to absorb virus particles at or nearly a neutral pH. Positive results of detection procedures will be confirmed by use of tissue culture when applicable or by restriction analysis and subsequent gel electrophoresis to visualize the viral constituents or metabolic products. This project is being supported under the NSF Program of Expedited Awards for Novel Research. Results of this project are expected to assist in determination of the impact that potential contamination of water by the HIV-virus can have on the engineering design of processes and systems for disinfection and decontamination of environmental resources.

该项目的目标是对用于检测和回收人类免疫缺陷病毒（HIV）的过滤技术以及使用基因探针技术检测与固体接触的水中是否存在这种病毒进行探索性研究。以及被这种病毒污染的液体废物。 在这项初步研究中，将通过使用带正电的深层过滤器来研究病毒的回收，该技术已被证明可以在中性或接近中性的 pH 值下吸收病毒颗粒。 检测程序的阳性结果将通过在适用时使用组织培养或通过限制性分析和随后的凝胶电泳来确认病毒成分或代谢产物。 该项目得到了 NSF 小说研究加急奖励计划的支持。 该项目的结果预计将有助于确定艾滋病病毒对水的潜在污染可能对环境资源消毒和净化过程和系统的工程设计产生的影响。