US-Belgium Cooperative Research: Regulation of Phage Mu Transposition

美国-比利时合作研究：噬菌体Mu转座的调控

• 批准号: 8821706
• 负责人: Norman Higgins
• 金额: $ 1.05万
• 依托单位: University of Alabama at Birmingham
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1993-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8821706&HistoricalAwards=false
• 关键词: US; Belgium; Cooperative; Research; Regulation

This award will support collaborative research between a group of U.S. and Belgian biochemists. The U.S. investigators are Dr. N. P. Higgins, University of Alabama at Birmingham and Dr. Martin Pato, University of Colorado Health Science Center. The Belgian researcher is Dr. Ariane Toussaint, Free University of Brussels. The long range goal of this project is to understand regulation of genetic transposition in bacterial populations. The Mu transposase and semi-essential genes participate in a highly efficient replication-transposition reaction in vivo. In this __ ____ project, experiments will be performed to analyze the transposase and early genes that influence Mu biology at the stages of the transcription and DNA synthesis. Objectives of the collaborative work include i) expression, ii) purification and iii) characterization of DNA binding proteins encoded by the virus. The investigators will extend joint efforts to chromosome-associated proteins (HU and other "histone-like" proteins) of E. coli and _ ____ Salmonella typhimurium. This approach will expand basic knowledge __________ ___________ of the chromosomal proteins that organize a functional folded nucleoid. The three investigators run laboratories that have demonstrated complementary expertise in all of the areas to be studied. Systems involving purified proteins for analyzing Mu transcription and replication in vitro are established in __ _____ Birmingham. Methods for analyzing Mu transcription and replication in vivo are established in Denver. The group in Brussels has genetic strategies for selecting mutants and carrying out the genetic dissection of proteins related to Mu biology. By coordinating experiments at the three sites, the investigators can test a hypothesis genetically and measure predicted behavior in ___ vitro and in vivo in a relatively short period of _____ _______ time.

该奖项将支持美国和比利时生物化学家之间的合作研究。 美国调查人员是阿拉巴马大学伯明翰大学的N. P. Higgins博士和科罗拉多大学健康科学中心的Martin Pato博士。 比利时的研究员是布鲁塞尔免费大学Ariane Toussaint博士。 该项目的远距离目标是了解细菌种群中遗传转位的调节。 MU转座酶和半必需基因参与体内高效的复制转置反应。 在这个__ ____项目中，将进行实验，以分析在转录和DNA合成阶段影响MU生物学的转座酶和早期基因。 协作工作的目标包括i）表达，ii）纯化和ii）表征由病毒编码的DNA结合蛋白。 研究人员将将联合努力扩展到大肠杆菌和_ ____ ____沙门氏菌的染色体相关蛋白（HU和其他“类似组蛋白”蛋白）。 这种方法将扩大基本知识__________ ________________________________________________________________________________________________________ 这三名调查人员经营实验室，这些实验室在要研究的所有领域都表现出了互补的专业知识。 ______伯明翰建立了涉及纯化的用于分析MU转录和复制的纯化蛋白的系统。 在丹佛建立了分析体内MU转录和复制的方法。 布鲁塞尔的该组具有选择突变体并进行与MU生物学相关的蛋白质的遗传解剖的遗传策略。 通过在三个地点进行协调实验，研究人员可以在______ _______时间相对较短的时间内测量遗传假设，并测量___体外和体内的预测行为。